nanoString GeoMx DSP User Manual page 53

Instrument
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MAN-10152-01 for software v2.5
Overlays, ROI Transfer, Export Images
ROI Transfer
ROI Transfer copies ROIs from an existing scan onto another scan. This feature is useful for:
Transferring ROIs from one section to a serial section.
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Creating ROI templates for large slide cohorts (to keep numbers, types, and
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labels of ROIs consistent).
Rescanning failed slides.
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Only scans with status Approved, Collected, Error, or Aborted can have ROIs transferred
from them, and only scans with status Scanned or Annotated can have ROIs transferred onto
them.
1. In the Scan Workspace, select the Overlays button to open the Overlays Panel
38).
2. Click Transfer to open the Select Scan window, and select the scan from which to copy ROIs.
Click OK.
3. The ROI Transfer process begins and progress is shown on the screen. The time to transfer
depends on the size of the scanned tissue and the number of ROI to be transferred.
4. An overlay including ROIs is displayed on the current scan. Adjust the overlay using the
tools Move, Scale, Resize, Rotate, or Mirror Image, to position the ROIs as you wish.
ROIs that are positioned outside the boundary of the destination scan will not transfer.
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ROIs that are resized and are now larger than the maximum or smaller than the minimum ROI
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size will not transfer.
A rectangle ROIs that is rotated will transfer as a 4-sided polygon.
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A circle ROI that is stretched to an ellipse will transfer as a circle.
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FOR RESEARCH USE ONLY. Not for use in diagnostic procedures.
GeoMx DSP Instrument User Manual
(see Figure
53

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