nanoString GeoMx DSP User Manual page 41

Instrument
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MAN-10152-01 for software v2.5
Select ROIs & Segment
Grid ROIs
1. Select the Grid ROIs tool in the Scan Workspace
2. Select the ROI shape (circle or rectangle), diameter, and column gap (distance between
each column of ROIs), row gap (distance between each row of ROIs), and odd row indent
(to indent every other row in a checkerboard pattern).
3. Click on the scan in the location where you
intend to place one corner of the grid and
drag. The grid area will appear as a yellow
dotted perimeter and the individual ROI
previews will appear inside with white dotted
line perimeters. The ROI count within the grid
will appear in the lower right corner of the grid
parameters field.
Note that these are previews; the ROIs will not be applied to the scan until you complete the task by
clicking the Apply button (next step).
4. When the preview appears as it should, click Apply. The ROIs will be applied to the scan.
5. Once you have placed the ROIs, they become separate ROIs that can be moved and altered
individually.
A maximum of 380 ROIs can be placed on a single scan.
FOR RESEARCH USE ONLY. Not for use in diagnostic procedures.
GeoMx DSP Instrument User Manual
(see Figure
27).
Figure 27: Grid ROIs tool
41

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