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GeoMx DSP Instrument User Manual
Select ROIs & Segment
Custom Polygon
1. Select the polygon button from the Scan Workspace
(see Figure
26).
2. Single-click where you would like one vertex of the polygon; drag your mouse and watch
a line grow from this vertex.
3. Single-click to create more vertices and watch the ROI form.
4. Click back on the original vertex to complete the ROI. This most recently applied ROI will
now have a number designation.
5. Click and drag a vertex to adjust the polygon in shape and size. If resizing causes
unexpected changes in polygon shape and size, create a new polygon, then return to the
polygon you wish to alter and it should resize properly.
6. Each side of a polygon has a center point; click on a center point to convert it to a vertex.
Click on a vertex to convert it to a center point.
For protein analysis, the area of a polygon ROI should be between between 2,500-40,000 µm
RNA analysis, the area of a polygon ROI should be greater than 40,000 μm
square ROI over or near your polygon ROI may help estimate its size. Open the ROIs List (see page
43) to check the dimensions of established ROIs.
Maximum ROI dimensions are 660 µm x 785 µm. A maximum of 380 ROIs can be placed on a single
scan.
FOR RESEARCH USE ONLY. Not for use in diagnostic procedures.
40
MAN-10152-01 for software v2.5
Figure 26: Scan Workspace
polygon button
2
. Placing a 40,000 µm
2
. For
2
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