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Maintenance
Changing or replacing the wash head
Changing or
replacing the
wash head
Checking the
liquid line
Checking the
residual volume
120
Thermo Scientific Wellwash Versa User Manual
Rinse both channels with deionized distilled water using the syringe
●
supplied with the instrument.
Refit the wash head plugs.
●
Attach the wash head to the instrument, prime the system and
●
ensure that liquid comes out evenly from all tips.
Caution Avoid breaking the smaller aspiration tube while detaching the
wash head. If the tube breaks, replace with a new one. Do not use any
other type of tubing.
If you change the wash head to another type, first change the wash head
type in the Settings menu and then calibrate the wash head.
If you replace the wash head to the same type, calibrate the new wash
head.
Refer to "Installing the wash head(s)" on page 18 and "Calibrate
current wash head" on page 97.
Caution Only use wash heads that have an identification label.
Check the liquid line for possible leaks. Ensure that the silicone tubes
are intact and there are no breaks or holes in them.
To ensure valve operation, first prime the system, then lift the buffer
bottle about 20 cm and finally ensure that the tips are not dripping.
Check the residual volume by dispensing 200 μl of 0.02% Tween™
solution over the whole plate. Aspirate and determine the residual
volume by weighing the plate.
Make a protocol with a Dispense and an Aspirate step:
1. Dispense: 200 μl, Dispense height end: 17.
2. Aspirate: Aspirate mode: Sweep2, Aspirate height: 2.6mm,
Aspirate speed: High, Aspirate time: 1s.
Tare weigh an empty plate using a laboratory balance. Select the whole
plate and run the protocol. Weigh the plate again and determine the
average residual volume per well in μl by dividing the mass in mg by 96.
Example: Residual liquid weight 91.2 mg. Residual volume/well [μl] =
91.2/96 [μl] = 0.95 [μl]
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Thermo Fisher Scientific
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Troubleshooting
