Mechanical Cleaning Of The Wash Head; Changing Or Replacing The Wash Head; Checking The Liquid Line; Checking The Residual Volume - Thermo Scientific Wellwash User Manual

Mechanical cleaning of the wash head

•
Detach the wash head from the tubing.
•
Remove the wash head plugs and use the declogging tool to clean the tips.
•
Rinse both channels with deionized distilled water using the syringe supplied with the
instrument.
•
Refit the wash head plugs.
•
Attach the wash head to the instrument, prime the system and ensure that liquid comes out
evenly from all tips.
Caution!
breaks, replace with a new one. Do not use any other type of tubing.

Changing or replacing the wash head

If you change the wash head to another type, first change the wash head type in the Settings menu
and then calibrate the wash head.
If you replace the wash head to the same type, calibrate the new wash head.
Refer to "Installing the wash head" and "Calibrate current wash head".
Caution!

Checking the liquid line

Check the liquid line for possible leaks. Ensure that the silicone tubes are intact and there are no
breaks or holes in them.
To ensure valve operation, first prime the system, then lift the buffer bottle about 20 cm and finally
ensure that the tips are not dripping.

Checking the residual volume

Check the residual volume by dispensing 200 µl of 0.02% Tween™ solution over the whole plate.
Aspirate and determine the residual volume by weighing the plate.
Make a protocol with dispense and aspirate step:
1. Dispense: 200 µl, Dispense height end: 17.
2. Aspirate: Aspirate mode: Sweep2, Aspirate height: 2.6mm, Aspirate speed: High, Aspirate
time: 1s.
Tare weigh an empty plate using a laboratory balance. Select the whole plate and run the protocol.
Weigh the plate again and determine the average residual volume per well in µl by dividing the mass
in mg by 96.
Example: Residual liquid weight 91.2 mg. Residual volume/well [µl] = 91.2/96 [µl] = 0.95 [µl]

Checking the dispense accuracy

Check the dispensing accuracy by dispensing one volume over the whole plate and determine the
volume by weighing the plate.
Create a dispense protocol which dispenses 300 µl over the whole plate. Tare weigh an empty plate
using a laboratory balance and dispense 0.02% Tween solution onto the plate. Weigh the plate again
and determine the average volume dispensed per well in µl by dividing the mass in mg by 96.
Example: Dispensed liquid weight 28.608 g. Volume/well [µl] = 28608/96 [µl] = 298 [µl]
Avoid breaking the smaller aspiration tube while detaching the wash head. If the tube
Only use wash heads that have an identification label.
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