Cell Aspirate - Thermo Scientific Wellwash Versa User Manual

Cell aspirate

Thermo Fisher Scientific
Aspirate speed – Define the aspirate rate as Low, Medium, or High.
●
The default is High in 96-well format and Low in 384-well format.
Aspirate time (s) – The aspirate time ranges from 0 s to 10 s in
●
1 s increments. The default is 1 s.
Caution A zero second aspirate time will increase the residual
volume. ▲
Strip over mode – Define the strip over function. The default is
●
No.
No – All selected strips are processed as fast as possible.
•
Yes – If Yes is selected, unused strips are processed dry. The
•
time parameters are measured during the first protocol run.
The wash head stays above unused strips for as long as it
takes to process each selected strip. This selection is
recommended when the timing for washing strips is critical.
You can maintain the time between cycles constant with this
option.
A Cell aspirate step removes liquid from the wells. Refer to "Cell wash"
on page 71.
The Cell aspirate step has the following parameters:
Aspirate height (mm) – The aspirate height ranges from 0 mm to
●
14 mm in 0.1 mm increments. The default height varies according
to the plate type and well shape (see Table 6–7 on page 72). Refer to
Figure 6–40.
Caution Too high an aspirate height increases the residual volume.
Caution Aspirating too low may disrupt the cells or block the
channels. ▲
Aspirate speed – Define the aspirate rate Low, Medium, or High.
●
The default is Low.
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