Cell Wash - Thermo Scientific Wellwash Versa User Manual

Thermo Fisher Scientific

Cell wash

Washing of cells can be carried out in 96-well plates only with a special
optional 2x8 / 96 (cell wash) wash head. The Cell wash step provides a
gentler wash than the normal Wash step. Refer to "Wash" on page 67.
Liquid is dispensed dropwise into and aspirated from the well during
washing of cells. Offsetting the dispense tips toward the side rather than
into the center of the well will dissipate the energy of the fluid stream
prior to reaching the cell layer.
When defining the aspiration parameters, it is advisable to leave a
residual. The residual fluid will act as a shield to prevent trauma to the
cell layer during the subsequent fluid dispense. It also has the added
advantage of maintaining cell hydration. The aspirate height has to be
set higher than the cell layer. Avoiding aspiration delays is also critical.
There is a significant amount of vortex turbulence in the region
immediately surrounding the aspiration tips, which has the potential to
be quite damaging to the cell layer.
The cell wash parameters are grouped into four columns. Use the
Right and Left arrow keys to move from one column to another.
The Cell wash step has the following parameters:
Buffer source – Define the buffer source channel A, B, or Rinse.
●
The default is A.
Wash volume (μl) – Define the volume of the wash buffer used in
●
microplate washing. The wash volume range is 50 μl to 1000 μl in
10 μl increments. The default value is 250 μl.
Menus, Tabs and Parameters
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