Intended Use; Principle Of Operation - Thermo Scientific Wellwash Versa User Manual

Introduction to the Wellwash Versa Microplate Washer

Intended use

Intended use
Principle of
operation
12 Thermo Scientific Wellwash Versa User Manual
The Wellwash Versa is a microplate washer intended for automated
washing, aspiration, dispensing, and shaking of 96- and 384-well plates
and strips that meet ANSI/SBS standards. The instrument can be
equipped with up to four liquid bottles. It can be used in research or
routine-test laboratories by professional personnel.
As the Wellwash Versa is part of an analyzing system for the end user,
the user is responsible for validation of the whole system to enable
production of reliable and safe results. If the assay performance is
essential to the analysis, the test result has to be assured using internal
quality controls or an alternative test.
It is recommended using Good Laboratory Practice (GLP) during the
analyzing process.
Use for self-testing is excluded.
The Wellwash Versa can be used to wash and prepare plates for a
variety of test routines, mainly in ELISA applications. The Wellwash
Versa (Figure 1-1) is a microplate strip washer for automated washing,
aspiration, dispensing, and shaking of both 96- and 384-well plate
formats: the 96-well plate in both landscape and portrait orientation,
and the 384-well plate in landscape orientation. The instrument is
equipped with three standard buffer bottles and one waste liquid bottle
each with a cap, tubing, and a liquid level sensor. The instrument has a
color display with an intuitive graphical user interface and keypad for
programming and controlling the instrument. The Wellwash Versa
comes with a 1x8, 2x8, 1x12, or 2x12 wash head. The plate carrier
places the microplate accurately under the wash head to perform the
desired operation. During priming the wash head moves into the
priming vessel to fill the liquid tubing from the liquid bottle to the
wash head tips.
The 96-well plates and strips are designed to be washed in 1x8, 2x8,
1x12, or 2x12 format, and the 384-well plates in 1x16 format (Figure
1–2). Additionally, a 2x8 cell wash head is available for washing of cells
in 96-well plates. The wash heads have both dispensing and aspiration
channels. In the washing step, the wash heads aspirate continuously and
descend until they reach the bottom of the well and the liquid is
aspirated (Figure 1–3). In sweep mode, the wash heads aspirate in two
positions of the well bottom to enable a low residual volume (Figure 1–
4). When the wash heads are in the upper position, the desired volume
is dispensed into the well (Figure 1–3 and Figure 1–4). Aspiration runs
continuously, enabling overflow dispensing of large volumes. After the
first row or column has been washed, the next row or column is moved
under the wash heads for washing.
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