Checking The Dispense Accuracy; Checking The Aspirate Height; Manual Priming - Thermo Scientific Wellwash Versa User Manual

Checking the
dispense
accuracy
Checking the
aspirate height

Manual priming

Thermo Fisher Scientific
Check the dispensing accuracy by dispensing one volume over the
whole plate and determine the volume by weighing the plate.
Create a dispense protocol which dispenses 300 μl over the whole plate.
Tare weigh an empty plate using a laboratory balance and dispense
0.02% Tween solution onto the plate. Weigh the plate again and
determine the average volume dispensed per well in μl by dividing the
mass in mg by 96.
Example: Dispensed liquid weight 28.608 g. Volume/well [μl] =
28608/96 [μl] = 298 [μl]
Check the aspirate height by aspirating at the 0.0 mm level.
Make a protocol with an Aspirate step: Aspirate mode: Normal,
Aspirate height: 0.0, Aspirate time: 10.
Disable the Plate sensor. Select the first column/strip and press Start
without a plate. The wash head moves to the 0.0 mm level. Ensure that
the wash head tips are on the plate bottom level of the plate carrier.
Refer to Figure 6–40. Enable the Plate sensor after the test.
If both the Prime and Boost prime functions fail, you have to perform
manual priming.
To prime a dry pump manually (Figure 11–46):
1. Fill the buffer bottle completely with liquid and close the cap. Place
the bottle at the same level as the instrument.
2. Disconnect the liquid bottle tube from the tube fitting on the
instrument.
3. Attach a 20 ml plastic syringe (Cat. No. N02942, supplied with the
instrument) to the free end of the tube.
4. Gently aspirate liquid from the liquid bottle to fill the tube by
"filling" the syringe. Leave a few centimeters of air at the end of the
tube to prevent spilling.
5. Close (pinch) the tube, continue pinching the tube and then
disconnect the tube from the syringe.
6. Reattach the tube to the fitting on the instrument side and release
your grip.

Checking the dispense accuracy

Thermo Scientific Wellwash Versa User Manual
Maintenance
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