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Thermo Fisher Scientific
Chapter 5
Running Protocols
1. Ensure that the buffer solutions in the bottles A, B, and Rinse are
correct and that there is sufficient liquid for the protocol. Also
ensure that the Waste bottle is not full when you begin. Ensure that
the liquid level sensors are enabled and symbols are green. Refer to
"Liquid level detection" on page 42. Ensure that the tubing is
properly fitted.
The liquid levels in the bottles are monitored during runs. Follow
the color-coded bottle icons on the info text bar. Refer to "Filling
and emptying liquid bottles" on page 44.
Ensure that the intake tube is fixed to the lowest position on the
cap, whereby no red tubing is visible above the cap, and fully
immersed in the buffer bottle.
2. Select a protocol. Refer to "Starting ready-made protocol" on page
44.
The selected protocol name is shown on the Protocol row in the
Main menu.
3. Ensure that the protocol parameters are correct.
4. Insert the 96- or 384-well plate to be washed onto the plate carrier
and ensure that the microplate is correctly oriented according to
the wash head configuration.
The A1 position of the plate is in the upper left corner when the
nx8 or 1x16 wash head is in use and in the lower left corner for the
nx12 wash head. Refer to "Loading the plate" on page 46.
If you are using strip plates, ensure that the individual strips are
properly attached to the plate frame.
Ensure that the plate is not covered.
5. Press the
button to prime the instrument. Refer to "Priming
PRIME
the system" on page 47.
6. Select the strips if they are not already selected. Refer to "Strip
selection" on page 48.
Thermo Scientific Wellwash Versa User Manual
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Table of Contents
Troubleshooting
