Thermo Scientific Wellwash Versa User Manual page 70

Menus, Tabs and Parameters
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68 Thermo Scientific Wellwash Versa User Manual
The wash parameters are grouped into two windows. Use the Right
and Left arrow keys to move from one window to another.
The Wash step has the following parameters:
Buffer source – You can define the buffer source channel A, B, or
●
Rinse. The default is A.
Wash volume (μl) – Define the volume of the wash buffer used in
●
microplate washing. For a 96-well plate, the range is 50 μl to
1000 μl in 10 μl increments. The default value is 300 μl.
For a 384-well plate, the range is 20 μl to 300 μl in 10 μl
increments. The default value is 100 μl.
Note It is possible to dispense more than the well volume and the excess
liquid is aspirated, as the aspiration is always on. If a so-called overflow
volume is selected where the dispensing volume is greater than the well
volume, pay attention to the dispense height. Check that the wash head
tips are inside the well.
Wash cycles – Define the number of wash cycles that will be
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performed, from 1 to 10. The default value is 3.
Soak time (mm:ss) – Define the time period between wash cycles in
●
the Wash step. The default value is 00:00 (= no soak). The
minimum value is 0 s and the maximum value 60 min in increments
of 1 s.
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