Prime - Thermo Scientific Wellwash Versa User Manual

Thermo Fisher Scientific
Soak time (mm:ss) – Define the time period between a wash cycle
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in microplate wash protocols. The minimum value is 0 s and the
maximum value 60 min in increments of 1 s. The default value is
00:01.
Shake speed –Define the shake speed Off, Low, Medium, or High.
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The default is Off. The shake speed setting is only effective when the
soak time is > 00:00.
Caution Ensure that the liquid does not spill at the shake speed and
volume settings in use.
Caution The instrument is not intended for shaking purposes only. A
separate microplate shaker is available if needed (e.g. iEMS
Incubator/Shaker). Refer to

Prime

The Prime step is performed to fill the liquid tubing completely from
the selectable buffer bottle intake tube to the wash head tips. A protocol
should have a Prime step before each buffer is used to ensure that the
liquid tubing is filled correctly with the desired liquid before performing
any Wash or Dispense steps.
The default priming volume is 30 ml in order to fill the tubing properly.
The dead volume of the liquid tubing is 20 ml (Figure 5-34).
Note Avoid unnecessary priming with air because it may damage the
pump. ▲
The Prime step has the following parameters:
Buffer source – Define the buffer source channel A, B, or Rinse.
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The default is A.
Prime volume (ml) – The prime volume ranges from 5 ml to
●
100 ml in 5 ml increments. The default is 30 ml.
Priming is carried out in the priming position. Aspiration is on while
priming. At the end, the priming vessel is emptied and the wash head
returns to the home position.
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