Thermo Scientific Wellwash Versa User Manual page 74

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72 Thermo Scientific Wellwash Versa User Manual
Note It is possible to dispense more than the well volume and the excess
liquid is aspirated. If a so-called overflow volume is selected where the
dispensing volume is greater than the well volume, the dispense height is
affected. That is, it is not possible to lift the wash head too high without
aspirating the excess liquid.
Wash cycles – Define the number of wash cycles that will be
●
performed, from 1 to 10. The default value is 3.
Soak time (mm:ss) – Define the time period between wash cycles in
●
the Cell wash step. The default value is 00:00 (= no soak). The
minimum value is 0 s and the maximum value 60 min in increments
of 1 s.
Shake speed – Define the shake speed Off, Low, Medium, or High.
●
The default is Off. The shake speed setting is effective only when the
soak time is > 00:00.
Caution Ensure that the liquid does not spill at the shake speed and
volume settings in use.
Wash mode – Define the wash mode. The default is Plate mode.
●
Plate – All selected strips are processed before the next cycle.
•
Strip – Each selected strip is processed the number of cycles
•
defined before moving to the next strip.
Aspirate height – The aspirate height ranges from 0 mm to 14 mm
●
in 0.1 mm increments. The default height varies according to the
plate type and well shape (Table 6–7). The zero height from which
the aspirate height is defined is the lowest level of a plate when the
plate is properly placed on the plate carrier. Refer to Figure 6–40.
Caution Too high an aspirate height increases the residual volume.
Note The aspirate height determines the amount of residual volume in
the wells. If you want the wells entirely empty, move the tips completely
to the well bottom or slightly below so that the wash head rests on the
bottom of the well. ▲
Table 6–7. Plate and well shape vs. default aspirate height
Plate / Well shape Default aspirate height
96 / Flat 6.1 mm
96 / C 6.1 mm
96 / U 6.1 mm
▲
▲
▲
Default aspirate heights are
defined so that the wash head
stays above the bottom. For
example, 3.0 mm above the
bottom with flat bottom plates.
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