Dispense - Thermo Scientific Wellwash Versa User Manual

Menus, Tabs and Parameters
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76 Thermo Scientific Wellwash Versa User Manual
Aspirate time (s) – The aspirate time ranges from 0 s to 10 s in
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1 s increments. The default is 1 s.
Aspirate offset – Define the aspirate offset from - 1.5 mm to
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1.5 mm in 0.1 mm increments. The default is -1.0 mm.
Wash head speed – This speed is used during the last 3 mm before
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the aspirate height is reached. The wash head speed ranges from
1 mm/s to 10 mm/s in 1 mm/s increments. The default is 10 mm/s.
Strip over mode – Define the strip over function. The default is
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No.

Dispense

A Dispense step fills the wells with liquid.
The Dispense step has the following parameters:
Buffer source – Define the buffer source channel A, B, or Rinse.
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The default is A.
Dispense volume (μl) – For a 96-well plate, the dispense volume
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range is 50 μl to 400 μl in 10 μl increments and the default value
300 μl.
For a 384-well plate, the dispense volume range is 20 μl to 120 μl in
10 μl increments and the default value 100 μl.
Dispense height start – The dispense height start ranges from
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0 mm to 20 mm in 0.1 mm increments. The default is 17.0 mm.
Ensure that the value fits the plate in use. Because dispensing starts
from a lower level in a well and ends at a higher level, the start
height should be lower than or equal to the end height. Refer to
Figure 6–40.
No – All selected strips are processed as fast as possible.
•
Yes – If Yes is selected, unused strips are processed dry. The
•
time parameters are measured during the first protocol run.
The wash head stays above unused strips for as long as it
takes to process each selected strip. This selection is
recommended when the timing for washing strips is critical.
You can maintain the time between cycles constant with this
option.
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