Thermo Scientific Wellwash Versa User Manual page 75

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Aspirate offset – Define the aspirate offset from - 1.5 mm to
1.5 mm in 0.1 increments. The default is -1.0 mm.
Aspirate speed – Define the aspirate rate Low, Medium, or High.
The default is Low.
Wash head speed – This speed is used during the last 3 mm before
the aspirate height is reached. The wash head speed ranges from
1 mm/s to 10 mm/s in 1 mm/s increments. The default is 10 mm/s.
Aspirate time (s) – The aspirate time ranges from 0 s to 10 s in
1 s increments. The default is 1 s.
Dispense height start – The dispense height start ranges from
0 mm to 17 mm in 0.1 mm increments. The default is 7.1 mm.
During dispensing, the wash head starts from a lower level in a well
(the so-called dispense height start) and rises towards the dispense
height end, as the liquid volume increases. The start height should
be lower than or equal to the end height. Ensure that the value fits
the plate in use. Refer to Figure 6–40.
Dispense height end – The dispense height end ranges from
0 mm to 17 mm in 0.1 mm increments. The default is 12.0 mm.
Refer to dispense height start above. Refer to Figure 6–40.
Note Because aspiration is on during dispensing, the end volume in the
well depends on the dispense height. If the liquid level in the well
during dispensing reaches the wash head tip, then excess liquid is
aspirated.
Note It is possible to dispense more than the well volume and the excess
liquid is aspirated, as the aspiration is always on during dispensing. If a
so-called overflow volume is selected where the dispense volume is
greater than the well volume, ensure that the dispense height end is not
outside the well.
Dispense offset – The dispense offset value changes the wash head
position horizontally, enabling a gentle dispensing via the well wall.
The dispense offset ranges from - 1.5 mm to 1.5 mm in 0.1 mm
increments. The default is 1.0 mm.
Caution Make sure that the liquid is dispensed into the well.
Dispense tip touch – The dispense tip touch movement is used to
remove the last droplet from the wash head tips after the dispensing
action. The dispense tip touch ranges from 0.0 mm to 1.5 mm in
0.1 mm increments. The default is 0.0 mm.
Final aspirate – The wells are emptied at the end of the step. The
default is Yes, which leaves wells empty. No leaves the wells wet.
Menus, Tabs and Parameters
Thermo Scientific Wellwash Versa User Manual
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