Thermo Scientific Wellwash Versa User Manual page 72

Menus, Tabs and Parameters
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70 Thermo Scientific Wellwash Versa User Manual
Note The aspirate height determines the amount of residual volume in
the wells. If you want the wells entirely empty, move the tips completely
to the well bottom or slightly below so that the wash head rests on the
bottom of the well. ▲
Table 6–6. Plate and well shape vs. default aspirate height
Plate / Well shape Default aspirate height
96 / Flat 2.6 mm
96 / C 2.9 mm
96 / U 4.2 mm
96 / V 5.4 mm
96 / Star 3.1 mm
384 / Flat 2.2 mm
Aspirate speed – Define the aspirate rate Low, Medium, or High.
●
The default is High.
Aspirate time (s) – The aspirate time ranges from 0 s to 10 s in 1 s
●
increments. The default is 1 s.
Dispense height start – The dispense height start ranges from
●
0 mm to 17 mm in 0.1 mm increments. The default is 6.0 mm.
Ensure that the value fits the plate in use. Because dispensing starts
from a lower level in a well and ends at a higher level, the start
height should be lower than or equal to the end height. Refer to
Figure 6–40.
Dispense height end – The dispense height end ranges from
●
0 mm to 17 mm in 0.1 mm increments. The default is 14.4 mm.
Refer to Figure 6–40.
Note Because aspiration is on during dispensing, the end volume in the
well depends on the dispense height. If the liquid level in the well
during dispensing reaches the wash head tip, then excess liquid is
aspirated. ▲
Note It is possible to dispense more than the well volume and the excess
liquid is aspirated, as the aspiration is always on during dispensing. If a
so-called overflow volume is selected where the dispense volume is
greater than the well volume, ensure that the dispense height end is not
outside the well. ▲
Final aspirate – The wells are emptied at the end of the step. The
●
default is Yes, which leaves wells empty. No leaves the wells wet.
Default aspirate heights are
defined so that the wash heads
rest on the bottom of the well.
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