Related Manuals for Promega GloMax
Summary of Contents for Promega GloMax
- Page 1 O P E R A T I N G M A N U A L GloMax Discover System ® Instructions for Use of Product GM3000 Revised 8/18 TM397...
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Page 2: Table Of Contents
5.G. Removing or Replacing Stainless Steel Tubing ..............86 6. Troubleshooting......................... 86 6.A. Error Messages ......................... 86 Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 www.promega.com TM397 · Revised 8/18... -
Page 3: Introduction
Figure 1. The GloMax Discover System. ® Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TM397 · Revised 8/18 www.promega.com... -
Page 4: Specifications
EU Declaration of Conformity for the product is available from Promega on request. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 www.promega.com... - Page 5 AFC (Ex: 405nm, Em: 495–505nm) Detection Limit 2fmol fluorescein/200µl Linear Dynamic Range 6 logs (assay-dependent) Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TM397 · Revised 8/18 www.promega.com...
- Page 6 Disk Space 64GB Other Requirements Microsoft.NET Framework 4.6.2, Microsoft Excel 2013 Professional ® Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 www.promega.com TM397 · Revised 8/18...
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Page 7: Product Components
GloMax® Discover Luminescence Filter Paddle 1 each GM3011 GloMax® Discover Fluorescence Filter Paddle 1 each GM3012 Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TM397 · Revised 8/18 www.promega.com... -
Page 8: Precautions And Special Instructions
Ne regardez pas directe- ment la lumière UV. Clean up spills. Nettoyer les déversements. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 www.promega.com TM397 · Revised 8/18... - Page 9 • Do not leave plate in the instrument after protocol is completed. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TM397 · Revised 8/18 www.promega.com...
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Page 10: Environmental Requirements
5–30°C, up to 75% humidity Operating Altitude Conditions less than 2,000 meters above sea level Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 www.promega.com TM397 · Revised 8/18... -
Page 11: Inspection And Unpacking
96-well aperture, Fluorescence Filter Paddle and Luminescence Filter Paddle are preinstalled on the instrument. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TM397 · Revised 8/18... - Page 12 After removing the red Packing Bracket, remove the blue Packing Bracket Mount, and ensure the instrument is free of any packaging material parts. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 www.promega.com...
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Page 13: Setting Up The Glomax ® Discover System
Connect the Instrument AC Power Adapter to the back of the instrument and to a power outlet. Note: We recommend connecting the instrument to an uninterrupted power supply (~500VA). Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TM397 · Revised 8/18... -
Page 14: Switching On The Glomax ® Discover Instrument
Discover software. The GloMax Discover System is now ready to use. ® Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 www.promega.com TM397 · Revised 8/18... -
Page 15: Shutting Down The Glomax ® Discover Instrument
Select one of the yellow Quick Read protocols on the ‘Home’ screen, or access a protocol of interest by pressing the Protocols button. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TM397 · Revised 8/18... - Page 16 ‘Home’ screen. Press the Analysis button to analyze results generated from the protocol (see Section 3.F, Analysis of Results). Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 www.promega.com...
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Page 17: Glomax ® Discover User Interface Screens
Exit Back Tools Door Help Figure 9. GloMax Discover ‘Home’ screen. ® Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TM397 · Revised 8/18 www.promega.com... -
Page 18: Quick Read Screens
Discover ‘Fluorescence Quick Read’ screen. On the plate map, blue wells are selected. ® Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 www.promega.com TM397 · Revised 8/18... - Page 19 Start measurement. igure 12. GloMax Discover ‘Absorbance Quick Read’ screen. ® Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TM397 · Revised 8/18 www.promega.com...
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Page 20: Protocol Screen
Remove button. The user will be asked to confirm the request to delete protocols. Figure 14. Delete Protocol message from the GloMax Discover software. ® Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 www.promega.com TM397 · Revised 8/18... - Page 21 Save, Save As and Start protocol. Figure 15. GloMax Discover ‘Protocol’ screen. ® Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TM397 · Revised 8/18 www.promega.com...
- Page 22 Discover ‘New Protocol’ screen showing drag and drop feature to create custom ® protocols. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 www.promega.com TM397 · Revised 8/18...
- Page 23 To run the protocol, press the Start button in the lower right corner of the screen. Figure 19. GloMax Discover New Protocol Successfully Saved user message. ® Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TM397 · Revised 8/18 www.promega.com...
- Page 24 However, the global plate map has columns 1–6 selected. When locking the protocol, each step will revert to the global plate map (columns 1–6). Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 www.promega.com...
- Page 25 They may be removed by selecting the black X at the top right corner of each function. Figure 22. GloMax Discover New Protocol Functions. ® Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TM397 · Revised 8/18 www.promega.com...
- Page 26 Enter between 1 second and 72 hours in increments of 1 second. Figure 24. Wait function. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 www.promega.com TM397 · Revised 8/18...
- Page 27 Note: The heater will continue to remain ON after the run unless it is turned OFF or the PC is disconnected from the instrument. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TM397 · Revised 8/18...
- Page 28 See Setting Up and Operating the Injector System (Section 4) for more details. Figure 27. Inject Function. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 www.promega.com...
- Page 29 Note: The instrument is not intended to be used as a long-term shaking incubator, and long-term shaking may damage the instrument. Figure 28. Shake Function. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TM397 · Revised 8/18 www.promega.com...
- Page 30 Figure 30. ‘Prompt’ screen with the Open Door option. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 www.promega.com TM397 · Revised 8/18...
- Page 31 Note: If adding an injector step inside a loop function, ensure that there is sufficient space in the well to accommodate multiple additions of reagent. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TM397 · Revised 8/18...
- Page 32 All well-associated operations within the kinetic block must have the same well selection. The plate maps of these operations are not individually editable. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 www.promega.com...
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Page 33: Running A Protocol
If multiple reads of the plate are performed, each result can be displayed by selecting the step that generated that result in the left navigation column. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TM397 · Revised 8/18... - Page 34 Users can use touch-screen gestures to zoom in or out, or to pan the well map. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 www.promega.com...
- Page 35 Figure 37. Example of individual well result for kinetic data. Figure 38. Results plate map for kinetic data. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TM397 · Revised 8/18...
- Page 36 ® or tap the Done button to return to the results plate map. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 www.promega.com TM397 · Revised 8/18...
- Page 37 ® Figure 40. Results export to Microsoft Excel workbook. ® Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TM397 · Revised 8/18 www.promega.com...
- Page 38 Templates and Analyses screen’. See Section 3.E, ‘Results, Templates and Analyses’ Screen for instructions on accessing saved results. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 www.promega.com...
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Page 39: Results, Templates And Analyses' Screen
Touch any of the list entries to view the associated results, template or analysis. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TM397 · Revised 8/18... - Page 40 (.template and .analysis) that can be imported into the GloMax ® software on another PC. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 www.promega.com TM397 · Revised 8/18...
- Page 41 Analyses’ screen, you can Export or Remove one or more list items, Import a result, template or analysis (.raw, .template, .analysis) file or create a New Template. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TM397 · Revised 8/18...
- Page 42 Figure 43. Importing Results, Templates or Analyses. Use the file browser screen to browse to the location of the desired import file. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 www.promega.com...
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Page 43: Analysis Of Results
Performing an analysis. • Editing the result graph. • Saving and exporting the analysis. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TM397 · Revised 8/18 www.promega.com... - Page 44 Opening a result file will take the user to the ‘Templates’ screen. Select the saved template from the list. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 www.promega.com...
- Page 45 Figure 45. Compute a ratio of a two-read result file, or select Multiple reads to analyze each read separately. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TM397 · Revised 8/18...
- Page 46 ‘Select Plate Type’ screen. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 www.promega.com...
- Page 47 Figure 47. New Template creation. From this screen the user will define the curve fit type, units, well types, concentrations/dilution factors and names in the analysis template. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TM397 · Revised 8/18...
- Page 48 No formula assigned Z´-Factor Calculates Z´-Factor Z´-Factor = 1 – |µ – µ Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 www.promega.com TM397 · Revised 8/18...
- Page 49 To select nonadjacent wells, touch a well and hold down until a rectangle appears, and continue with selection. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TM397 · Revised 8/18...
- Page 50 This blank option is applicable to Ratiometric reads from NanoBRET™ assays. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 www.promega.com...
- Page 51 Unknown wells containing replicates of individual samples. STAND name has been applied to the replicates in the standard curve, and BLANK has been assigned to the Blank wells. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TM397 · Revised 8/18...
- Page 52 (100 for wells A1–D8, 200 for wells E1–H8). A volume of 1µl has been assigned to the Unknown wells. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 www.promega.com...
- Page 53 H12) and Group A applied. Up to 11 groups may be selected at a time. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TM397 · Revised 8/18...
- Page 54 Group drop-down menu. A unique color will be applied to each group. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 www.promega.com...
- Page 55 Figure 53. Select the icon in the lower right corner of each Group to edit the group label. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TM397 ·...
- Page 56 Figure 54. Type a new name for the Group label. Edited text will appear in the Group drop-down menu and in the graph legend. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 www.promega.com...
- Page 57 % Control or Fold Change. The Normalize button becomes active once a Control has been assigned to the plate. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TM397 · Revised 8/18...
- Page 58 “Analysis has successfully completed” message will appear on the screen. Select OK to view the analyzed results. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 www.promega.com...
- Page 59 The graphing tab will remain inactive until the Run button has been pressed. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TM397 · Revised 8/18...
- Page 60 After the analysis has been run, touch the Graphing Tab on the right side of the screen to view the graph of the results. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 www.promega.com...
- Page 61 Touch Reset View to return the graph to the default view. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TM397 · Revised 8/18...
- Page 62 Outliers can be excluded by touching the outlier point in the Data Tab, and then touching the Exclude button. After excluding outliers, touch the Run button to reanalyze the data with outliers removed. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 www.promega.com...
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Page 63: Settings' Screen
Discover’ screen located on the Settings menu. This figure provides example ® information only. Your instrument information may vary. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TM397 · Revised 8/18 www.promega.com... - Page 64 The Heater will remain on after the run unless manually turned OFF by the user or the PC is disconnected from the instrument. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 www.promega.com...
- Page 65 To change filter slides for BRET/filtered luminescence or for fluorescence (including FRET), select the appropriate Reconfigure... button. Figure 64. Initial screen showing instrument filter configuration. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TM397 · Revised 8/18 www.promega.com...
- Page 66 Tap Next to complete the reconfiguration. Figure 66. Filter configuration procedure (part 1). Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 www.promega.com TM397 · Revised 8/18...
- Page 67 Figure 68. Filter configuration procedure (part 3). Figure 69. Filter configuration procedure (part 4). Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TM397 · Revised 8/18...
- Page 68 Remove the Excitation module by unscrewing the thumb screw. The excitation module should slide forward so that you can remove it. Tap Next to continue. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 www.promega.com TM397 · Revised 8/18...
- Page 69 Figure 73. Replacing the hatch door. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TM397 · Revised 8/18...
- Page 70 Figure 74. Aperture loading instructions screen. Figure 75. Aperture Plate with 384-well Aperture Fitting in the front position. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 www.promega.com...
- Page 71 Figure 77. ‘Export Folder’ screen. Select a folder to which logs will be exported. Press Save to export log files. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TM397 ·...
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Page 72: Administrator Settings
This button is only visible if the current user has Administrator-level access to the GloMax software. ® Figure 78. ‘Administrator Page’ screen. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 www.promega.com TM397 · Revised 8/18... - Page 73 Once a folder path is selected, press the Save button to save the new path or Cancel to exit the screen without saving. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TM397 · Revised 8/18...
- Page 74 Figure 80. Specifying Export Folder path. Use the Select Folder button to specify an export folder path for export files. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 www.promega.com...
- Page 75 Figure 81. Analysis Settings tab. Specify the file formats for export of analyses and the export folder location for templates and analyses. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TM397 · Revised 8/18...
- Page 76 Note: Changing the instrument name will force a reboot of the Tablet PC. Figure 83. Settings Administrator screen Instrument Name. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 www.promega.com...
- Page 77 Cancel to return to the ‘Administrator Page’ screen without importing files. Figure 84. Settings Administrator screen Import Protocols. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TM397 · Revised 8/18...
- Page 78 The context-dependent Help Screen can be accessed from any of the menu screens. Figure 86. Help Screen. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 www.promega.com...
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Page 79: Setting Up And Operating The Injector System (Optional)
Figure 88. Positioning the Injector System on top of the GloMax Discover System. ® Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TM397 · Revised 8/18 www.promega.com... - Page 80 Figure 92. Installation of the inlet tube holder clip. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 www.promega.com TM397 · Revised 8/18...
- Page 81 Instrument and the Tablet PC. Power the Instrument back on, then turn on the Tablet PC. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TM397 · Revised 8/18 www.promega.com...
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Page 82: Operating The Injector System
Note: The reagent container holder can accommodate four sizes of containers: a round flat-bottom bottle, a 50ml conical tube, a 15ml conical tube and a 15ml test tube. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 www.promega.com... - Page 83 Once the injectors are primed, they are ready for use according to the programmed protocol. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TM397 · Revised 8/18...
- Page 84 Note: Remove Waste Collection Tray when priming and flushing are complete. See Section 5.D for injector cleaning instructions. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 www.promega.com...
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Page 85: Cleaning And Maintenance
Tablet PC. Don’t use window cleaner or other chemical cleaners on the Tablet PC. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TM397 · Revised 8/18... -
Page 86: Cleaning The Injectors
Discard the used injector tubing assembly. Twist the fitting of the replacement tubing into the correct port of the injector pump. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 www.promega.com... -
Page 87: Removing Or Replacing Stainless Steel Tubing
See Reference position loss See Reference position loss (Underrun) position loss (Underrun) Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TM397 · Revised 8/18 www.promega.com... - Page 88 Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 www.promega.com TM397 · Revised 8/18...
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Page 89: Common Problems
The luminescent output of the sample is saturating the detector. Use less sample. Use a black plate. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TM397 · Revised 8/18 www.promega.com... -
Page 90: Use Of A Usb Flash Drive
Antivirus software will slow the performance of the Tablet PC and will affect the performance of the GloMax ® Instrument while it is measuring samples. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 www.promega.com TM397 · Revised 8/18... - Page 91 Close the ‘Control Panel’ screen by tapping the red X button in the upper right corner of the screen. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TM397 ·...
- Page 92 Figure 98. The ‘Windows Settings’ screen. The ‘Windows Settings’ screen provides access to multiple functions that can be used to configure options and settings for the Tablet PC. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 www.promega.com...
- Page 93 Make sure this switch is in the on position if your location observes daylight savings time during the summer hours. Otherwise make sure the switch is in the off position. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TM397 · Revised 8/18...
- Page 94 • Select and run preloaded protocols • Build user-defined protocols • View, export results Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 www.promega.com TM397 · Revised 8/18...
- Page 95 Panel B. Touching the lusrmgr.msc item from the search list opens the ‘Local Users and Groups (Local)’ screen. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TM397 · Revised 8/18...
- Page 96 Close the ‘Computer Management’ screen. Figure 103. Adding users to the PromegaAdministrators group. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 www.promega.com TM397 · Revised 8/18...
- Page 97 Swipe in from the right edge of the screen and select Search, then type in “lusrmgr.msc” and press Enter. The Local Users and Groups item should appear. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TM397 · Revised 8/18...
- Page 98 PromegaUsers group. Select the user to be removed and tap the Remove… button Touch OK to remove the selected user from the PromegaUsers group. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 www.promega.com...
- Page 99 Figure 107. ‘Instrument Name’ screen. Administrators can manually enter the instrument name from the ‘Instrument Name’ screen. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TM397 · Revised 8/18...
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Page 100: Icons And Symbols
Press to turn the heat map display “on” or “off. Delete a result file from the Results Screen. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 www.promega.com... -
Page 101: Warranty And Service
Decontamination (see Section 7.D). If the instrument has been exposed to any chemical, biological or radioactive hazards, contact Promega Technical Services for decontamination instructions before shipping. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TM397 · Revised 8/18... -
Page 102: Certificate Of Decontamination
This instrument is certified by the undersigned to be free of radioactive contamination. Date: ____________________________________ Place: ____________________________________ Signature: _________________________________ Name (block capital letters): ________________________________________________________ Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 www.promega.com TM397 · Revised 8/18... -
Page 103: Related Products
10ml G8091 ® Apo-ONE Homogeneous Caspase-3/7 Assay* G7792 ® *Additional Sizes Available. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TM397 · Revised 8/18 www.promega.com... - Page 104 ONE-Glo™ + Tox Luciferase Reporter and Cell Viability Assay* 1 plate E7110 *Additional Sizes Available Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 www.promega.com TM397 · Revised 8/18...
- Page 105 300 assays (384-well plate) V1501 GloSensor™ cAMP Reagent* 25mg E1290 *Additional Sizes Available. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TM397 · Revised 8/18 www.promega.com...
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Page 106: Summary Of Changes
Product claims are subject to change. Please contact Promega Technical Services or access the Promega online catalog for the most up-to-date information on Promega products. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 www.promega.com...