Promega GloMax Operating Manual page 49

3.F. Analysis of Results (continued)
Creating a New Analysis Template (continued)
Well Type Descriptions:
Unknown: A well where the concentration of the sample is unknown. It is possible to set a name, dilution factor
and volume for this type of well.
Known: A well where the concentration of the sample is known. You can set a name and concentration for this
type of well.
Blank: A well containing a blank for the experimental data set. The average values of all blank wells will be
subtracted from all Known, Unknown and Control wells prior to analysis.
Control: Two controls may be selected, indicated as Control 1 and Control 2. Both Controls are required for
Z´-Factor analysis. Only one control may be used for % Control and Fold-Change data normalization.
6. If the data to be analyzed with this analysis template is NanoBRET™ data, check the NanoBRET x1000
box to automatically apply the mBRET multiplier to the ratiometric NanoBRET™ data.
Touch the Units text box to open a keyboard and specify the unit of measurement for concentration on the
7.
entire plate. Only one unit of concentration can be specified for the plate. Select Insert in the lower right
corner of the keyboard to access a list of Greek letters and characters, fractions or other commonly used
scientific labels.
8. Touch and drag to select wells on the plate map that will contain samples of a given type. From the Well
Type drop-down box select the corresponding well type (Unknown, Known, Blank, Control) that you
wish to assign to those wells (Figure 48). For any Unknown wells, the user also may enter a sample volume.
To select nonadjacent wells, touch a well and hold down until a rectangle appears, and continue with
selection.
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TM397 · Revised 8/18 www.promega.com