Common Problems - Promega GloMax Operating Manual

6.B. Common Problems
Symptoms
Injectors sputter or drip
Injectors not injecting
Plate stuck or jammed in instrument
Tablet PC touch screen does not appear to be working 1.
Power failure during run
Instrument problem: "door opened"
message: explanation
Luminescent signal is reported as SAT
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TM397 · Revised 8/18
Causes and Comments
Reagent buildup in injector lines. Run the flushing
protocol (Section 4.B).
Injector speed may not be optimal for the viscosity of the
solutions used.
No reagent in injector lines. Confirm that the inlet tubing is
inserted into the bottom of the reagent container.
Reprime the injectors. (Section 4.B).
Injector tubing is clogged and may need to be replaced.
(Section 5.F)
Press Door, press Self Test on the 'Settings' screen or
press Recovery in the Tools menu (Section 3.G). Any of
these methods can be use to attempt to recover the plate.
If the issue is not resolved, contact Promega Technical
Services (techserv@promega.com).
Verify the plug is securely connected to the Tablet PC.
2. Verify that the instrument is plugged into the USB
port on the Tablet PC.
3. Restart the Tablet PC, and launch the GloMax
Discover software.
4. If the issue is not resolved, contact Promega Technical
Services (techserv@promega.com).
In the event of a power failure, turn OFF the instrument
and the Tablet PC. When power is resumed, turn the
instrument and Tablet PC back ON. The results that were
generated during the run (before the power failure) will be
saved.
1. Instrument door was opened during a run,
causing the reading to abort. Restart the run.
2. Something prevented the instrument door from
fully closing as the Multiwell Plate Sample Tray
was retracting. Check the instrument door for
any obstructions.
The luminescent output of the sample is saturating the
detector.
1. Use less sample.
2. Use a black plate.
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