Promega Maxwell 16 MDx Operating Manual
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Promega Maxwell 16 MDx Operating Manual

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O P E R A T I N G M A N U A L
Maxwell
®
MDx Instrument
Instructions for Use of Product
AS3000
16
2800 Woods Hollow Rd.
Madison, WI USA
Revised 4/15
TM320

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Summary of Contents for Promega Maxwell 16 MDx

  • Page 1 O P E R A T I N G M A N U A L Maxwell ® MDx Instrument Instructions for Use of Product AS3000 2800 Woods Hollow Rd. Madison, WI USA Revised 4/15 TM320...

  • Page 2: Table Of Contents

    7.A. Changing the Hardware ......................... 33 7.B. Changing the Instrument Firmware Configuration ................36 Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 www.promega.com TM320 · Revised 4/15...
  • Page 3 12. Certificate of Decontamination ....................61 13. Summary of Changes ........................62 Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TM320 · Revised 4/15 www.promega.com...

  • Page 4: Maxwell

    Sample Track is a communications program that allows downloading and printing of the run data (see Technical Manual #TM314). Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 www.promega.com...

  • Page 5: Maxwell ® 16 Mdx Instrument Specifications

    15.5mm, 4.5W, 0.17A current, 29V, Spectral Peak 253.7, UV output 0.8W Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TM320 · Revised 4/15 www.promega.com...

  • Page 6: Product Components And Symbol Key

    Catalog number Manufacturer Serial number 2800 Woods Hollow Rd. Madison, WI USA Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 www.promega.com TM320 · Revised 4/15...

  • Page 7: Inspection

    Panel B. LEV Cartridge Rack, LEV High-Strength Magnetic Rod, Plunger Bar Adapter. Panel C. SEV Cartridge Rack, SEV Elution Rack, SEV Magnetic Rod. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TM320 · Revised 4/15...

  • Page 8: Precautions

    Please follow your institutional requirements for disposal of the accessories. 6687TA.eps Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 www.promega.com TM320 · Revised 4/15...
  • Page 9 Equipment can be hazardous due to the use of chemical and biohazardous substances Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TM320 · Revised 4/15...

  • Page 10: Environmental Requirements (Operating, Shipping And Storage Conditions)

    Do not use near flammable gases or liquids. • Do not place near other electrically sensitive instruments. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 www.promega.com TM320 · Revised 4/15...

  • Page 11: Hardware Overview

    Run/Stop buttons allow navigation between screens. The Up and Down buttons are for navigation within a screen. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TM320 ·...
  • Page 12 Figure 5. Communications ports on the side of the instrument. This side view shows the communication ports for the instrument. The USB port is used to attach the bar code reader. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 www.promega.com...
  • Page 13 (rack not shown). Figure 6. Magnetic assemply components and platform. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TM320 · Revised 4/15 www.promega.com...

  • Page 14: Hardware Mode

    When the machine is powered up after the installation, the installed hardware will match the hardware mode displayed on the Home screen. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 www.promega.com...

  • Page 15: Unpacking And Setting Up The Maxwell ® 16 Mdx Instrument

    Upper shipping anchors Figure 8. Upper shipping anchors. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TM320 · Revised 4/15 www.promega.com...

  • Page 16: Uv Bulb Installation

    16 MDx Instrument is now ready for operation. ® Figure 10. UV bulb in the power supply. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 www.promega.com TM320 · Revised 4/15...

  • Page 17: Maxwell ® Sample Track Software

    The platform, plunger bar and magnetic rod assembly are moved to check operation, and the UV light is briefly switched on to ensure that the bulb is functioning. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TM320 · Revised 4/15...
  • Page 18 Figure 11. The Select Languages screen. Once you have selected a language, press the forward arrow button. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 www.promega.com...
  • Page 19 The “Touch Screen Reset Successful” message indicates that the calibration was successful, and the Wizard can continue. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TM320 · Revised 4/15...
  • Page 20 Figure 15. UV Settings screen. Select the desired option on the UV screen, and then set the time for UV treatment. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 www.promega.com...
  • Page 21 Figure 17. Unit Name screen. Touching the screen to name an instrument will open a keyboard to add a unique identifier. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TM320 · Revised 4/15...
  • Page 22 PINs are added, then select the Exit button in the User Setup Complete screen. Select No to end the Wizard. A maximum of 10 users can be added. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 www.promega.com...
  • Page 23 User Protocol (Section opens the Maxwell 16 Configuration screen. ® 10.A). Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TM320 · Revised 4/15 www.promega.com...

  • Page 24: Operating The Maxwell ® 16 Mdx Instrument

    Please contact Promega Technical Services at techserv@promega.com for more information on these specialized options. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 www.promega.com TM320 · Revised 4/15...
  • Page 25 Please contact Promega Technical Services at techserv@promega.com for more information on these specialized options. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TM320 · Revised 4/15...

  • Page 26: Setting Up Samples And Collecting Input Data

    Important. There are two bar codes on the kit label. Scan the bar code at the top of the label. You will receive an error message if you scan the wrong bar code. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 www.promega.com...
  • Page 27 The bar code data will appear on the screen next to the Eluate ID box. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TM320 · Revised 4/15...
  • Page 28 For LEV methods, once all of the data have been verified, place the cartridge rack into the instrument, close the door, and press the Run/Stop button. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 www.promega.com...
  • Page 29 For LEV runs, once all of the data have been verified, place the cartridge rack into the instrument, and press the Run/Stop button. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TM320 · Revised 4/15...

  • Page 30: Performing A Sample Purification Run

    Calibration Error. Figure 27. The Processing screen. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 www.promega.com...

  • Page 31: Post-Run Uv Light Treatment

    UV light treatment to avoid damage to the nucleic acid. You may cancel the UV treatment at any time. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TM320 · Revised 4/15...

  • Page 32: Run Reports

    The Print option opens the Select File screen where files can be selected for printing. Figure 30. The Select File screen. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 www.promega.com...

  • Page 33: Data File Transfer At A Later Time

    16 Configuration screen. Here you can choose the parameter that you ® wish to change. Maxwell Figure 31. The ® Configuration screen. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TM320 · Revised 4/15 www.promega.com...

  • Page 34: Changing The Hardware

    LEV Plunger Adaptor is even with the blue arm and seated flat along its full length. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 www.promega.com...
  • Page 35 Gently lift the LEV Magnetic Rod Assembly, and remove it. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TM320 · Revised 4/15 www.promega.com...
  • Page 36 Running the instrument when the hardware mode shown does not match the physical hardware installed will damage the instrument. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 www.promega.com...

  • Page 37: Changing The Instrument Firmware Configuration

    Changing the Sample Tracking Settings. Selecting the Sample Tracking option in the Configuration screen allows you to change the sample tracking options. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TM320 · Revised 4/15...

  • Page 38: Cleaning And Maintenance

    Section 8.F, or contact Promega Technical Services for assistance. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 www.promega.com...

  • Page 39: Cleaning The Hardware

    Once the instrument has been UV-treated, the UV time may be reset by navigating to the UV Settings screen and resetting the UV treatment time. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TM320 · Revised 4/15...

  • Page 40: Replacing The Uv Bulb

    Belts: Inspect the belts periodically. If excessive wear or excessive slack is noted, contact Promega or your local Promega representative, and arrangements will be made to service the instrument. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 www.promega.com...

  • Page 41: Troubleshooting

    If you still experience problems after recalibration, contact Promega or your local Promega representative for service. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TM320 · Revised 4/15...
  • Page 42 It should be replaced. See Section 8.E. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 www.promega.com...
  • Page 43 Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TM320 · Revised 4/15...
  • Page 44 If the calibration error persists, please contact Promega or your local Promega representative for service. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 www.promega.com...
  • Page 45 UV lamp comes on as part of the startup diagnostic tests. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TM320 · Revised 4/15...

  • Page 46: Appendix

    Also, elution buffer could evaporate during long, heated elution times. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 www.promega.com...
  • Page 47 1.50 minutes 3.00 minutes 3.00 minutes 7.00 minutes 2.00 minutes 0 minutes Cycles Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TM320 · Revised 4/15 www.promega.com...
  • Page 48 In the User Protocols screen, select a number, then press the the Run/Stop button. Figure 34. User Protocol screen. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 www.promega.com...
  • Page 49 Figure 36. Editing run parameters. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TM320 · Revised 4/15...
  • Page 50 10 existing custom methods, more custom methods can only be added by changing an existing method. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 www.promega.com...
  • Page 51 DNA, RNA or Protein (SEV) or DNA, RNA or Total NA (LEV). Select the parameter to be changed, and change as described above. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TM320 · Revised 4/15...
  • Page 52 The user-defined methods may be run by selecting User in the Protocols screen (see Section 5.A). Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 www.promega.com...

  • Page 53: B Homogenization Method

    If heating was used during the homogenization method, allow the elution tube slots to cool before setting up the purification run. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TM320 · Revised 4/15...

  • Page 54: Updating Firmware

    Utilities Menu screen. Figure 44. Utilities Menu screen. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 www.promega.com TM320 · Revised 4/15...
  • Page 55 These are the parameters that can be changed in your facility. For any other service, see the Warranty information in Section 11. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TM320 · Revised 4/15...

  • Page 56: Instrument Return

    By hand, gently lower the plunger bar and magnetic rod assembly. Replace the Magnetic Rod/Plunger Bar shipping anchor thumbscrews and tighten by hand (Figure 8). Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 www.promega.com...

  • Page 57: Instrument Disposal

    Contact your local Promega Representative for disposal of the instrument. Please follow your institutional requirements to handle the disposal of accessories. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TM320 · Revised 4/15...

  • Page 58: Warranties, Service Agreements And Related Products

    Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 www.promega.com...

  • Page 59: Warranty And Service Agreement Options

    Additionally, it includes one annual preventive maintenance visit per year. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TM320 · Revised 4/15...
  • Page 60 The Installation and Operational Qualification package includes all of the components from both SA1001 and SA1011 in one service product. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 www.promega.com...

  • Page 61: Related Products

    DNA IQ ™ Reference Sample Kit for Maxwell 48 preps AS1040 ® Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TM320 · Revised 4/15 www.promega.com...

  • Page 62: Certificate Of Decontamination

    This instrument is certified by the undersigned to be free of radioactive contamination. Date: Place: Signature: Name (block capital letters): Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 www.promega.com TM320 · Revised 4/15...

  • Page 63: Summary Of Changes

    Product claims are subject to change. Please contact Promega Technical Services or access the Promega online catalog for the most up-to-date information on Promega products. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TM320 · Revised 4/15...

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