Promega GloMax Operating Manual page 32

5. Rearrange the steps by touching and dragging the appropriate steps.
Figure 32. Loop function with detection modes selected.
Kinetics Function
The Kinetics function is used to perform a set of steps on a per-well basis, then repeating the set of steps a
desired number of times (i.e., inject and read one well multiple times before proceeding to the next well).
Note: Kinetics can be used with all detection modes, plate types, and protocol functions except for heat, shake and
user prompt functions. If shaking and/or heating is desired in a kinetic protocol, they must be applied to the entire
plate and therefore be placed outside of the kinetic step. Loops may not be placed within a kinetic step, but kinetic
steps may be placed within Loops. A maximum of 250 kinetic reads in 1-unit increments can be performed within
a single kinetic step. For example, 10 readings with a 1-second interval means that 1 reading is taken every
1 second for 10 seconds.
1. Add the Kinetics step to the protocol by dragging and dropping the Kinetics icon onto the protocol.
Figure 33. Kinetics function.
2. All well-associated operations within the kinetic block must have the same well selection. The plate maps of
these operations are not individually editable.
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TM397 · Revised 8/18