Promega GloMax Operating Manual page 52

As the template is created, replicates are identified using a combination of Well Type, Name,
Concentration/Dilution Factor and Volume. The number in the upper right corner signifies the replicate
number for that well.
10. Select all wells that have the same dilution factor or volume. Once wells are selected, touch the Concentration
or Dilution text box and assign the concentration or dilution factor value to the selected wells (Figure 50).
For Unknowns, input a Volume (in µl) that represents sample input volume in the well. For example, if 1µl
of sample volume was placed into the well, 1µl volume should be entered in the Volume (µl) box.
Note: The Clear button can be applied if a mistake is made during template creation. Clearing a well
removes all well type, naming, concentration/dilution factor and volume information associated with the
well.
Figure 50. Concentrations and dilution factors. In this figure, concentrations have been applied to the
standard curve replicates (shown at the bottom of each well). Dilution factors have been applied to the Unknowns
(100 for wells A1–D8, 200 for wells E1–H8). A volume of 1µl has been assigned to the Unknown wells.
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TM397 · Revised 8/18