Promega Maxwell 16 Operating Manual
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Promega Maxwell 16 Operating Manual

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Maxwell
Operating Manual
PRINTED IN USA. 2/08
16 Clinical Instrument
®
In Vitro Diagnostic
Medical Device
MDSS GmbH
Schiffgraben 41
30175 Hannover, Germany
INSTRUCTIONS FOR
USE OF PRODUCT
A S 2 0 5 0
Part# TM300

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Summary of Contents for Promega Maxwell 16

  • Page 1 Maxwell 16 Clinical Instrument ® Operating Manual INSTRUCTIONS FOR USE OF PRODUCT MDSS GmbH In Vitro Diagnostic A S 2 0 5 0 Schiffgraben 41 Medical Device 30175 Hannover, Germany PRINTED IN USA. 2/08 Part# TM300...

  • Page 2: Table Of Contents

    General Troubleshooting ..........................20 Power Failure ..............................21 Error Messages ..............................22 Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · Phone 608-274-4330 · Fax 608-277-2516 · www.promega.com Printed in USA. Part# TM300...
  • Page 3 ® Instrument Accessories and Purification Kits for Research and Forensic Use ........33 Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · Phone 608-274-4330 · Fax 608-277-2516 · www.promega.com Part# TM300 Printed in USA.

  • Page 4: Introduction

    Maxwell 16 Blood DNA Purification Kit (Cat.# AS1015). ® Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · Phone 608-274-4330 · Fax 608-277-2516 · www.promega.com Printed in USA. Part# TM300...

  • Page 5: Maxwell ® 16 Blood Dna Purification Procedure

    The temperature of samples is regulated by a heating system that is controlled by the protocol. The extracted nucleic acid can be used for PCR amplification. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · Phone 608-274-4330 · Fax 608-277-2516 · www.promega.com Part# TM300 Printed in USA.

  • Page 6: Maxwell ® 16 Instrument Specifications

    Dimensions (W × D × H): 325.5 × 438.2 × 326.5mm Power Requirements: 100–240VAC, 50–60Hz, 2.1A Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · Phone 608-274-4330 · Fax 608-277-2516 · www.promega.com Printed in USA. Part# TM300...

  • Page 7: Product Components And Symbol Key

    Important Catalog number Manufacturer Serial number Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · Phone 608-274-4330 · Fax 608-277-2516 · www.promega.com Part# TM300 Printed in USA. Page 6 2/08...

  • Page 8: Inspection

    16 Magnetic Elution Rack; E. Power Cable; F. Maxwell ® ® Cartridge Rack. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · Phone 608-274-4330 · Fax 608-277-2516 · www.promega.com Printed in USA. Part# TM300 2/08...

  • Page 9: Precautions

    It is recommended that the user evaluate the electromagnetic environment of the instrument prior to operation. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · Phone 608-274-4330 · Fax 608-277-2516 · www.promega.com Part# TM300 Printed in USA.
  • Page 10 Equipment can be hazardous due to the use of chemical and biohazardous substances. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · Phone 608-274-4330 · Fax 608-277-2516 · www.promega.com Printed in USA.

  • Page 11: Environmental Requirements

    RS-232 Cable to obtain firmware upgrades. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · Phone 608-274-4330 · Fax 608-277-2516 · www.promega.com Part# TM300 Printed in USA.
  • Page 12 ® and Maxwell 16 platform. ® Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · Phone 608-274-4330 · Fax 608-277-2516 · www.promega.com Printed in USA. Part# TM300 2/08 Page 11...

  • Page 13: Unpacking And Setting Up The Maxwell ® 16 Instrument

    Important: Save the shipping anchor thumbscrews in case the instrument needs to be returned for service or repair at a later date. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · Phone 608-274-4330 · Fax 608-277-2516 · www.promega.com Part# TM300 Printed in USA.

  • Page 14: Operational Mode Setup

    Connecting to the serial port on the back of the instrument is not required. Store the RS-232 Cable in a location near the instrument for future use. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · Phone 608-274-4330 · Fax 608-277-2516 · www.promega.com Printed in USA.

  • Page 15: (Ii) Hardware Configuration And Operational Mode Setup Requirements

    Failure to use the operational mode required for your selected hardware configuration will cause damage to the instrument. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · Phone 608-274-4330 · Fax 608-277-2516 · www.promega.com Part# TM300 Printed in USA.

  • Page 16: (Iii) Setting The Maxwell ® 16 Instrument Operational Mode

    Once the language is selected press “Run/Stop”, and then return to Menu. The display should now be in your selected language. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · Phone 608-274-4330 · Fax 608-277-2516 · www.promega.com Printed in USA.

  • Page 17: Operating The Maxwell ® 16 Instrument

    Tech. Manual #TM300 for troubleshooting for troubleshooting Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · Phone 608-274-4330 · Fax 608-277-2516 · www.promega.com Part# TM300 Printed in USA. Page 16...

  • Page 18: Sample Purification

    Use aerosol-resistant pipet tips when transferring samples to minimize the potential for cross-contamination. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · Phone 608-274-4330 · Fax 608-277-2516 · www.promega.com Printed in USA.

  • Page 19: Periodic Cleaning And Maintenance

    • If any of the hardware accessories need to be cleaned (i.e., cartridge or elution racks), wipe them with a cloth dampened with deionized water or 70% ethanol. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · Phone 608-274-4330 · Fax 608-277-2516 · www.promega.com Part# TM300 Printed in USA.

  • Page 20: Removal Of Magnetic Rod Assembly

    Promega representative, and arrangements will be made to service the instrument. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · Phone 608-274-4330 · Fax 608-277-2516 · www.promega.com Printed in USA.

  • Page 21: Troubleshooting

    If plungers are consistently left on, contact Promega or your local Promega representative for service. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · Phone 608-274-4330 · Fax 608-277-2516 · www.promega.com Part# TM300 Printed in USA.

  • Page 22: Power Failure

    Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · Phone 608-274-4330 · Fax 608-277-2516 · www.promega.com Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · Phone 608-274-4330 · Fax 608-277-2516 · www.promega.com Printed in USA.

  • Page 23: Error Messages

    “Press Run/Stop key” “Waiting!” “Heating the Block” Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · Phone 608-274-4330 · Fax 608-277-2516 · www.promega.com Part# TM300 Printed in USA. Page 22...

  • Page 24: Appendix I: Firmware Updates, Instrument Return, And Warranty Information

    Promega or your local Promega representative for replacement packaging. Repack the instrument according to the following instruction Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · Phone 608-274-4330 · Fax 608-277-2516 · www.promega.com Printed in USA.

  • Page 25: Instrument Disposal

    Contact your local Promega Representative for disposal of the instrument. Please follow your institutional requirements to handle the disposal of accessories. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · Phone 608-274-4330 · Fax 608-277-2516 · www.promega.com Part# TM300 Printed in USA.

  • Page 26: Certificate Of Decontamination

    Date: Place: Signature: Name (block capital letters): Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · Phone 608-274-4330 · Fax 608-277-2516 · www.promega.com Printed in USA. Part# TM300 2/08 Page 25...

  • Page 27: Warranty Information

    Repair service, if needed, will be billed at a flat rate to be agreed upon in advance. Your invoice will include shipping. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · Phone 608-274-4330 · Fax 608-277-2516 · www.promega.com Part# TM300 Printed in USA.

  • Page 28: Appendix Ii: Research And Forensic Applications Of The Maxwell ® 16 System

    Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · Phone 608-274-4330 · Fax 608-277-2516 · www.promega.com Printed in USA.
  • Page 29 Low Elution Volume hardware, select “LEV”. 2.LEV Hardware Press “Run/Stop” to select. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · Phone 608-274-4330 · Fax 608-277-2516 · www.promega.com Part# TM300 Printed in USA.
  • Page 30 Rsch = Research Fnsc = Forensic IVD = Clinical Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · Phone 608-274-4330 · Fax 608-277-2516 · www.promega.com Printed in USA. Part# TM300...

  • Page 31: Reconfiguring The Maxwell ® 16 Instrument Hardware

    Unscrew the three thumbscrews on top of the magnetic rod assembly. Thumbscrew Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · Phone 608-274-4330 · Fax 608-277-2516 · www.promega.com Part# TM300 Printed in USA.
  • Page 32 SEV to LEV will cause damage to the instrument. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · Phone 608-274-4330 · Fax 608-277-2516 · www.promega.com Printed in USA.
  • Page 33 Failure to change the instrument firmware operational mode from LEV to SEV will cause damage to the instrument. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · Phone 608-274-4330 · Fax 608-277-2516 · www.promega.com Part# TM300 Printed in USA.

  • Page 34: Instrument Accessories And Purification Kits For Research And Forensic Use

    48 preps AS1060 ® Sufficient for 48 automated isolations. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · Phone 608-274-4330 · Fax 608-277-2516 · www.promega.com Printed in USA. Part# TM300 2/08...
  • Page 35 16 Magnetic Elution Rack (for use with standard configuration) AS1202 ® Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · Phone 608-274-4330 · Fax 608-277-2516 · www.promega.com Part# TM300 Printed in USA.
  • Page 36 Patent Pending. U.S. Pat. Nos. 6,027,945, 6,368,800 and 6,673,631, Australian Pat. No. 732756, European Pat. No. 1 204 741 and Mexican Pat. No. 209436 have been issued to Promega Corporation for methods of isolating biological target materials using silica magnetic particles and simultaneous isolation and quantitation of DNA. Other patents are pending.
  • Page 37 Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · Phone 608-274-4330 · Fax 608-277-2516 · www.promega.com Part# TM300 Printed in USA. Page 36 2/08...
  • Page 38 A F 9 T M3 0 0 0 2 0 8 T M3 0 0 Promega Corporation • 2800 Woods Hollow Road • Madison, WI 53711-5399 USA Telephone 608-274-4330 • Fax 608-277-2601 w w w . p r o m e g a . c o m...

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