Table of Contents Introduction ....................4 1.1 Safety Symbols and Markings ............... 4 1.2 Physical Components ..................6 1.3 Computer and Power Connection ..............6 1.4 Shutter Mechanism ..................7 1.5 Cross-talk Reduction Grid ................7 1.6 Status Indicator ....................8 1.7 Readout Orientation ..................9 2 T echnical Specifications ................10 Product Components ................12 MyGlo™...
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All technical literature is available at: www.promega.com/protocols/ Visit the website to verify that you are using the most current version of this Operating Manual. Email Promega Technical Services if you have questions on use of this system: techserv@promega.com MyGlo™ Reagent Reader | TM732 | 5/24...
Introduction The MyGlo™ Reagent Reader is a device that measures light output using applications accessed via the cloud-based Promega ProNect™ Data Platform. The reader and data platform control, read and analyze samples assessed by compatible Promega assays in a standard 96-well microplate.
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Symbols Explanation Symbols Explanation Part Number Manufacturer Serial Number Date of Manufacture (Year-Month-Day) Consult your local Promega CE Compliance Mark Representative regarding Promega Corporation Madison, WI USA 53711 instrument disposal WEEE Directive (European Community Directive 2012/19/EU on Waste Electrical and Electronic 6687TA.eps...
Introduction Physical Components Your MyGlo™ Reagent Reader device has two major components: the Detection System and the light shield plate. The Detection System contains all electronic components, including the detectors. The light shield plate has been designed to hold a standard 96-well microplate. When the Detection System and light shield plate are closed, the device prevents ambient light from affecting read accuracy.
Introduction Shutter Mechanism Your MyGlo™ Reagent Reader Detection System is designed with a mechanical mechanism that opens and closes multiple times during a normal measurement to monitor and measure background signal for each well. Figure 2. Diagram of shutter mechanism. Cross-talk Reduction Grid Your MyGlo™...
Introduction Status Indicator Your MyGlo™ Reagent Reader is equipped with a status indicator that is easily visible to users. The status indicator depicts various states as shown in Figure 3. Color Appearance Status Name Status Description Scanning Self test Self test in progress. Solid Idle Instrument is idle and not currently reading a plate.
Introduction Readout Orientation To learn more about the read orientation specific to your MyGlo™ Reagent Reader-compatible Promega assay, visit www.promega.com/myglo-reagent-reader Figure 4. Panel A. Top readout orientation. Panel B. Bottom readout orientation. MyGlo™ Reagent Reader | TM732 | 5/24...
This instrument complies with the requirements of the EU Directives for Electromagnetic compatibility (EMC Directive) and electrical safety (the LVD). Compliance with the relevant Directives was demonstrated by third party compliance Testing to the appropriate EN and IEC standards. The product bears the CE mark on its label as evidence of compliance, and an EU Declaration of Conformity for the product is available from Promega upon request. Weight 570g/1.25lbs Dimensions 98 ×...
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Technical Specifications Performance Linear Dynamic Range 6 logs Cross-Talk Average <0.02% Sensitivity www.promega.com/myglo-reagent-reader Microplate Types www.promega.com/myglo-reagent-reader Readout Orientation www.promega.com/myglo-reagent-reader MyGlo™ Reagent Reader | TM732 | 5/24...
Product Components P R O D U C T S I Z E C A T. # MyGlo™ Reagent Reader 1 each MG1000 For Research Use Only. Includes: • MyGlo ™ Reagent Reader • USB-C to USB-A cable • AC Power Adapter and Power Supply •...
We recommend creating a ProNect™ user account before starting the instrument setup process. An account is necessary to activate and control the MyGlo™ Reagent Reader device. To create your ProNect™ user account, visit pronect.promega.com 1. Download and install the Connect2ProNect desktop application from www.promega.com/c2pmyglo.
Your MyGlo™ Reagent Reader is operated using MyGlo™ Reagent Reader-compatible ProNect™ applications that are accessible from your ProNect™ Data Platform dashboard. 1. Go to pronect.promega.com, log in to your ProNect™ account and select a MyGlo™ Reagent Reader-compatible ProNect™ application. 2. Each application is designed for specific Promega assays.
5.2 Standard MyGlo™ Reagent Reader Application Workflow For details on using MyGlo™ Reagent Reader-compatible applications, visit www.promega.com/pronect#apps. There are plate map guidelines, data analysis calculations and other information available for each ProNect™ application. Designing a Plate Map When using MyGlo™ Reagent Reader-compatible applications, you can design a plate map with well-specific parameters that are customized based on the Promega assay. Plate maps can be...
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Reagent Reader LED will blink yellow to indicate that you can remove your plate. Analyzing Your Data • Data visualizations have been customized for your MyGlo™ Reagent Reader-compatible Promega assay. • Data visualizations are generated and viewable once the measurement is complete. •...
Cleaning and Maintenance Your MyGlo™ Reagent Reader device has no user-serviceable parts. Opening the Detection System will void the warranty. Failure to observe the warnings in Section 1.1 may result in damage to the MyGlo™ Reagent Reader. This can reduce the lifetime and performance of the device. Cleaning the Exterior The exterior surface of your MyGlo™...
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Troubleshooting If you are unable to resolve any issues related to your MyGlo™ Reagent Reader or any additional questions not addressed here, please contact Promega Technical Services or your local Promega Branch Office or Distributor. Contact information is available at: www.promega.com. Email: techserv@promega.com Note: Promega Technical Services may request log files to help diagnose the problem. Follow the instructions for malfunctioning device below.
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Troubleshooting Issue Description Comments MyGlo™ Reagent Your MyGlo™ Reagent Reader • Ensure your MyGlo™ Reagent Reader offline is operated by a connected Reader is connected to the power ProNect™ account via Wi-Fi. adapter. When the MyGlo™ Reagent • Ensure that Wi-Fi signal is Reader is disconnected from available and the MyGlo™...
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Troubleshooting Issue Description Comments Moving your MyGlo™ You need to change the Wi-Fi To update your Wi-Fi configuration, Reagent Reader connection for your MyGlo™ connect your MyGlo™ Reagent to a new Wi-Fi Reagent Reader. Reader to your computer using the connection provided USB cable. •...
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To generate log files, connect device experiences issues not related your MyGlo™ Reagent Reader to a to WiFi connection or self-test computer with the provided USB failure, contact Promega cable. Technical Services. They • Open the Connect2ProNect may request log files to help application and log in using your diagnose the problem.
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Troubleshooting Issue Description Comments Plate detection error Plate detection failures happen There are two options to override a or failure when a MyGlo™ Reagent plate detection error or failure: Reader is in Ready mode and • To force a read, turn or hold the fails to detect a plate.
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The warranty does not include repair or replacement necessitated by accident, neglect, misuse, unauthorized repair or modification of the instrument. If a problem arises with the instrument, contact Promega Technical Services for support. Contact information is available at: www.promega.com. Email: techserv@promega.com. If further action is required, you will be given repair options and assigned a return authorization number.
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Products may be covered by pending or issued patents or may have certain limitations. Please visit our website for more information. Product claims are subject to change. Please contact Promega Technical Services or access the Promega online catalog for the most up-to-date information on Promega products.
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