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Summary of Contents for Promega Maxwell 16 MDx
- Page 1 Maxwell 16 MDx Instrument ® Technical Manual INSTRUCTIONS FOR USE OF PRODUCT AS3000-SC AS3000-LC PRINTED IN USA. 9/09 Part# TM320...
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Page 2: Table Of Contents
E. Replacing the UV Bulb F. Periodic Maintenance 9. Troubleshooting 10. Appendix A. User-Defined Methods B. Homogenization Method Promega Corporation 2800 Woods Hollow Road • Madison, WI 53711-5399 USA part# TM320 Toll Free in USA 800-356-9526 • Phone 608-274-4330 rev. 9/09... -
Page 3: Introduction
LEV mode with the SEV hardware • Maxwell ® Sample Track Software and bar code reader supplied separately. included Promega Corporation 2800 Woods Hollow Road • Madison, WI 53711-5399 USA part# TM320 Toll Free in USA 800-356-9526 • Phone 608-274-4330 rev. 9/09... -
Page 4: Inspection
16 MDx Instrument, please inspect the package carefully to make sure all accessories are present and that the instrument has not been damaged in shipping. If any item is damaged, contact Promega Technical Services. Standard accessories are shown in Figures 1 and 2. -
Page 5: Precautions
Do Warning. Biohazard. not operate the UV lamp if the instrument door is open. • Use only Promega supplied UV bulbs (Cat.# SP1080). • If the equipment is used in a manner other than that Warning. UV light hazard. Do... -
Page 6: Hardware Overview
Figure 6. Communications ports on the side of the instrument. This side view shows the communication ports for the instrument. The USB port is used to attach the bar code reader. Promega Corporation 2800 Woods Hollow Road • Madison, WI 53711-5399 USA part# TM320 Toll Free in USA 800-356-9526 •... - Page 7 For LEV, the cartridge sample rack containing the LEV cartridges is placed on the platform (rack not shown). Promega Corporation 2800 Woods Hollow Road • Madison, WI 53711-5399 USA part# TM320 Toll Free in USA 800-356-9526 • Phone 608-274-4330...
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Page 8: Hardware Mode
2. Remove the foam packaging from the sides of the instrument, and remove the clear plastic cover. Promega Corporation 2800 Woods Hollow Road • Madison, WI 53711-5399 USA part# TM320 Toll Free in USA 800-356-9526 • Phone 608-274-4330... -
Page 9: Uv Bulb Installation
® 16 MDx replace the bulb at that point. Replacement bulbs can be Instrument and print (see Section 6). purchased from Promega (Cat.# SP1080). E. Operational Setup Using the Touch Screen Note: In the instructions below, screen names are presented in bold type, and on-screen options are presented in italics. - Page 10 Selecting each of the screen options opens a keypad to add numerical values. Promega Corporation 2800 Woods Hollow Road • Madison, WI 53711-5399 USA part# TM320 Toll Free in USA 800-356-9526 • Phone 608-274-4330...
- Page 11 "Yes", the Select Users screen opens. The screen is initially populated with numbers. Select a number and then press the Run/Stop button. Promega Corporation 2800 Woods Hollow Road • Madison, WI 53711-5399 USA part# TM320 Toll Free in USA 800-356-9526 • Phone 608-274-4330...
- Page 12 Maxwell Sample Track software all PIN-protected options. If you lose your (see Section 6), or it allows deletion of run data from the Adminstrative PIN, contact Promega Technical Services. instrument. Selecting Setup opens the Maxwell ® 16 Configuration To add a PIN, select Edit/Add PIN in the User Settings screen.
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Page 13: Operating The Maxwell ® 16 Mdx Instrument
Select DNA to access the protocols for Blood and Cells (use with the Maxwell ® 16 Blood DNA ® Purification Kit Promega Cat.# AS1010 or Maxwell 16 Cell DNA Purification Kit, Cat.# AS1020), Buffy Coat (use with the Maxwell ® 16 Blood DNA Purification Kit Promega Cat.# AS1010) or Tissue... -
Page 14: Setting Up Samples And Collecting Input Data
® 16 Tissue LEV Total RNA Kit, Promega Cat.# AS1220), and cells (the Maxwell ® Cell LEV Total RNA Purification Kit, Promega Cat.# AS1225). • Select Viral to purify Viral total nucleic acid from serum or plasma (use with the Maxwell ®... - Page 15 Eluate ID box. the Barcode Input screen displays the information that can be collected. The report data available depend on the Promega Corporation 2800 Woods Hollow Road • Madison, WI 53711-5399 USA part# TM320 Toll Free in USA 800-356-9526 •...
- Page 16 Next Sample to navigate to the required sample, and Clear All option. re-enter all of the bar code information. Promega Corporation 2800 Woods Hollow Road • Madison, WI 53711-5399 USA part# TM320 Toll Free in USA 800-356-9526 • Phone 608-274-4330...
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Page 17: Performing A Sample Purification Run
Continuing the run will not damage the Maxwell ® 16 MDx Instrument but may result in suboptimal recovery of nucleic Promega Corporation 2800 Woods Hollow Road • Madison, WI 53711-5399 USA part# TM320 Toll Free in USA 800-356-9526 • Phone 608-274-4330... -
Page 18: Post-Run Uv Light Treatment
If Yes is selected, the File Output screen opens. Promega Corporation 2800 Woods Hollow Road • Madison, WI 53711-5399 USA part# TM320 Toll Free in USA 800-356-9526 • Phone 608-274-4330... -
Page 19: Data File Management
16 Configuration screen. 2. Follow the on-screen instructions. First, open the door. Figure 35. Adding the LEV Plunger Bar Adaptor. Promega Corporation 2800 Woods Hollow Road • Madison, WI 53711-5399 USA part# TM320 Toll Free in USA 800-356-9526 • Phone 608-274-4330... - Page 20 Figure 39. Installing the SEV Magnetic Rod Assembly. 8. Secure the SEV Magnetic Rod Assembly with the three thumbscrews, and hand-tighten. Promega Corporation 2800 Woods Hollow Road • Madison, WI 53711-5399 USA part# TM320 Toll Free in USA 800-356-9526 • Phone 608-274-4330...
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Page 21: Changing The Instrument Firmware Configuration
Configuration screen, and select the required language. The display will change over to the selected language. Promega Corporation 2800 Woods Hollow Road • Madison, WI 53711-5399 USA part# TM320 Toll Free in USA 800-356-9526 • Phone 608-274-4330... -
Page 22: Cleaning The Hardware
Belts: Inspect the belts periodically. If excessive wear or In case of spills in the instrument where there is a potential excessive slack is noted, contact Promega or your local biohazard, wipe up the spill with towels and wash the spill... -
Page 23: Troubleshooting
Platform shipping anchors have been removed. • Sensors may need to be cleaned. Contact Promega or your local Promega representative for assistance with sensor cleaning. • Look for obstructions that may be preventing a head from moving through its full range of motion (for example, plungers in the tracks of the deck). - Page 24 Heads that are not moving freely may be lubricated with light machine oil. Do not get oil on the drive belts. Use a small amount of oil on a cotton swab. If this does not correct the problem, contact Promega or your local Promega representative for service.
- Page 25 When I close (or open) the door, the There may be a problem with the door sensor. Please contact Promega program does not advance. or your local Promega representative for service.
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Page 26: Appendix
To update the firmware you must use the supplied 1Gb SD card. Other not successful. 1Gb SD cards may work, but Promega does not support other SD cards. It is recommended that you leave the SD card in its slot when not in use to prevent losing it. - Page 27 1.50 minutes 3.00 minutes 1.50 minutes 3.00 minutes 3.00 minutes 7.00 minutes 2.00 minutes 0 minutes Cycles Promega Corporation 2800 Woods Hollow Road • Madison, WI 53711-5399 USA part# TM320 Toll Free in USA 800-356-9526 • Phone 608-274-4330 rev. 9/09...
- Page 28 Number of Cycles. Select the down arrow, and add the new value using the keypad. The maximum number of cycles that can be added is 3; the minimum is 1. Promega Corporation 2800 Woods Hollow Road • Madison, WI 53711-5399 USA part# TM320 Toll Free in USA 800-356-9526 •...
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Page 29: Homogenization Method
Both the SEV and LEV modes have an option that allows homogenization of tissue samples prior to running the purification method. These methods must be user-validated Promega Corporation 2800 Woods Hollow Road • Madison, WI 53711-5399 USA part# TM320 Toll Free in USA 800-356-9526 • Phone 608-274-4330... -
Page 30: Updating Firmware
Maxwell ® 16 MDx Instrument port. Download the updated firmware from the Promega web site as main.bin onto a computer. Place the SD card in the computer’s SD card port, and download the updated firmware to the SD card. If you do not have an SD card reader on your computer,... -
Page 31: Service
Figure 54. Utilities Menu screen. Promega or your local Promega representative for support. If further action is required, repair options will be presented In the Utilities Menu screen, select Service Report to view and a return authorization number assigned if necessary. -
Page 32: Instrument Disposal
16 MDx Instrument will be free from removed from the instrument platform. defects in materials and workmanship for a period of one year from the date of delivery. Promega agrees, as its sole • Back up the data on the instrument by exporting the... -
Page 33: Warranty And Service Agreement Options
Maxwell ® 16 MDx Instrument Technical Manual • Shipping to and from Promega will be paid by Promega In order to keep your instrument at peak performance, pursuant to directions to be provided. The instrument Promega recommends that the Maxwell ®... -
Page 34: Certificate Of Decontamination
All prices and specifications are subject to change without prior notice. Product claims are subject to change. Please contact Promega Technical Services or access the Promega online catalog for the most up-to-date information on Promega products. © 2009 Promega Corporation. All Rights Reserved. - Page 35 This instrument is certified by the undersigned to be free of radioactive contamination. Date: Place: Signature: Name (block capital letters): Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · Phone 608-274-4330 · Fax 608-277-2516 · www.promega.com Printed in USA. Part# TM 0 ...
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