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Summary of Contents for Promega E8051
- Page 1 T e c h n i c a l M a n u a l GloMax ® -Multi Detection System INSTRUCTIONS FOR USE OF PRODUCTS E8031, E8041, E8051, E8061, E9031, E9061. PRINTED IN USA. Revised 5/10 Part# TM311...
- Page 2 All technical literature is available on the Internet at: www.promega.com/tbs/ Please visit the web site to verify that you are using the most current version of this Technical Manual. Please contact Promega Technical Services if you have questions on use of this system. E-mail: techserv@promega.com 1.
- Page 3 H. Disconnecting from a PC...................56 I. USB Flash Drive....................56 Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA Toll Free in USA 800-356-9526 · Phone 608-274-4330 · Fax 608-277-2516 · www.promega.com Part# TM311 Printed in USA. Page 2...
- Page 4 Each detection mode has dedicated optics for the Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA Toll Free in USA 800-356-9526 · Phone 608-274-4330 · Fax 608-277-2516 · www.promega.com Printed in USA. Part# TM311...
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Page 5: A. Inspection
Failure to do so may damage the electronics. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA Toll Free in USA 800-356-9526 · Phone 608-274-4330 · Fax 608-277-2516 · www.promega.com Part# TM311 Printed in USA. -
Page 6: C. Precautions
Check the tips periodically and replace as needed to avoid leaks onto the Microplate Sample Tray Cover. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA Toll Free in USA 800-356-9526 · Phone 608-274-4330 · Fax 608-277-2516 · www.promega.com Printed in USA. Part# TM311... -
Page 7: Product Components
-Multi+ UV-Visible Absorbance Module 1 each E9061 Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA Toll Free in USA 800-356-9526 · Phone 608-274-4330 · Fax 608-277-2516 · www.promega.com Part# TM311 Printed in USA. Page 6 Revised 5/10... -
Page 8: Hardware Overview
Figure 3. Rear view of the GloMax ® -Multi+ Detection System Instrument. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA Toll Free in USA 800-356-9526 · Phone 608-274-4330 · Fax 608-277-2516 · www.promega.com Printed in USA. Part# TM311 Revised 5/10... -
Page 9: Touch Screen Basics
Icon turns dark when files have been saved to the USB flash drive. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA Toll Free in USA 800-356-9526 · Phone 608-274-4330 · Fax 608-277-2516 · www.promega.com Part# TM311 Printed in USA. -
Page 10: B. Buttons On The Read Screen
• “Shaking” button. • “Dispense” button. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA Toll Free in USA 800-356-9526 · Phone 608-274-4330 · Fax 608-277-2516 · www.promega.com Printed in USA. Part# TM311 Revised 5/10 Page 9... -
Page 11: C. Buttons On The Results Screen
• Delete files and delete protocols button. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA Toll Free in USA 800-356-9526 · Phone 608-274-4330 · Fax 608-277-2516 · www.promega.com Part# TM311 Printed in USA. Page 10... -
Page 12: D. Buttons On The Tools Screen
PC hard drive. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA Toll Free in USA 800-356-9526 · Phone 608-274-4330 · Fax 608-277-2516 · www.promega.com Printed in USA. Part# TM311... -
Page 13: E. Terminology Used In Parameter Settings
Wavelength: Selects one or two Absorbance filters from a list. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA Toll Free in USA 800-356-9526 · Phone 608-274-4330 · Fax 608-277-2516 · www.promega.com Part# TM311 Printed in USA. -
Page 14: Home Screen
Figure 5. “Home” button. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA Toll Free in USA 800-356-9526 · Phone 608-274-4330 · Fax 608-277-2516 · www.promega.com Printed in USA. Part# TM311... -
Page 15: Protocol Management
Note: Once a plate format has been selected, this format will become the default setting for all subsequent selections. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA Toll Free in USA 800-356-9526 · Phone 608-274-4330 · Fax 608-277-2516 · www.promega.com Part# TM311 Printed in USA. - Page 16 Figure 7. Defining a New Protocol screen. Figure 8. Setting Fluorescence Options screen. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA Toll Free in USA 800-356-9526 · Phone 608-274-4330 · Fax 608-277-2516 · www.promega.com Printed in USA. Part# TM311...
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Page 17: B. Modifying A Saved Protocol
Before running any protocol, always verify the wells to be read. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA Toll Free in USA 800-356-9526 · Phone 608-274-4330 · Fax 608-277-2516 · www.promega.com Part# TM311 Printed in USA. -
Page 18: C. Using The Protocol Composer To Set Up Multiple Read Formats
1. Select and drag Protocol Option icons onto the Protocol Composer (Figure 10). Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA Toll Free in USA 800-356-9526 · Phone 608-274-4330 · Fax 608-277-2516 · www.promega.com Printed in USA. Part# TM311... - Page 19 Figure 11. Defining a multiple parameter protocol. Figure 12. Defining user prompt message screen. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA Toll Free in USA 800-356-9526 · Phone 608-274-4330 · Fax 608-277-2516 · www.promega.com Part# TM311 Printed in USA.
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Page 20: Glomax ® -Multi+ Detection System With Shaking
Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA Toll Free in USA 800-356-9526 · Phone 608-274-4330 · Fax 608-277-2516 · www.promega.com Printed in USA. Part# TM311... -
Page 21: A. Selecting Shaking Options
Figure 14. Selecting the Shaking parameters. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA Toll Free in USA 800-356-9526 · Phone 608-274-4330 · Fax 608-277-2516 · www.promega.com Part# TM311 Printed in USA. -
Page 22: Glomax ® -Multi+ Detection System With Temperature Control
Figure 15. Accessing the Heater option via the “Tools” button. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA Toll Free in USA 800-356-9526 · Phone 608-274-4330 · Fax 608-277-2516 · www.promega.com Printed in USA. Part# TM311... -
Page 23: B. Setting Incubation Parameters Followed By Plate Read
9. Touch the “Door” button again to retract the sample plate back into the instrument. 10. Press the “Start” button. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA Toll Free in USA 800-356-9526 · Phone 608-274-4330 · Fax 608-277-2516 · www.promega.com Part# TM311 Printed in USA. Page 22... -
Page 24: C. Setting Incubation Parameters Without Plate Read
Heater option on the Tools screen and do not select the Temperature Control button. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA Toll Free in USA 800-356-9526 · Phone 608-274-4330 · Fax 608-277-2516 · www.promega.com Printed in USA. Part# TM311... -
Page 25: Plate Format Selection
Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA Toll Free in USA 800-356-9526 · Phone 608-274-4330 · Fax 608-277-2516 · www.promega.com Part# TM311 Printed in USA. -
Page 26: B. Well Selection
Figure 19. Plate Mapping screen. On the plate mapping screen, green indicates selected wells, gray indicates deselected wells. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA Toll Free in USA 800-356-9526 · Phone 608-274-4330 · Fax 608-277-2516 · www.promega.com Printed in USA. Part# TM311... -
Page 27: C. Selecting Reference Wells (Absorbance Mode Only)
Figure 20. Selecting Reference wells (Ref), as seen in Absorbance mode. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA Toll Free in USA 800-356-9526 · Phone 608-274-4330 · Fax 608-277-2516 · www.promega.com Part# TM311 Printed in USA. -
Page 28: D. Changing The Optical Crosstalk Mask For 384-Well Plates
Note: Do not touch the bottom of the optical head. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA Toll Free in USA 800-356-9526 · Phone 608-274-4330 · Fax 608-277-2516 · www.promega.com Printed in USA. Part# TM311... - Page 29 Figure 24. Slide the 384-well optical crosstalk mask into place on the optical head. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA Toll Free in USA 800-356-9526 · Phone 608-274-4330 · Fax 608-277-2516 · www.promega.com Part# TM311 Printed in USA.
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Page 30: E. Well Scan Mode
6-well plates (left) and for 12-, 24- and 48-well plates (right). Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA Toll Free in USA 800-356-9526 · Phone 608-274-4330 · Fax 608-277-2516 · www.promega.com Printed in USA. Part# TM311... -
Page 31: F. Viewing Multiple Read Results For 6- To 48-Well Plates
“Door” button. The Microplate Sample Plate Cover may be in the way. Important: Close the instrument door immediately after each use. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA Toll Free in USA 800-356-9526 · Phone 608-274-4330 · Fax 608-277-2516 · www.promega.com Part# TM311 Printed in USA. -
Page 32: Luminometer Operation
After PMT activation begins, the “Start” button appears. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA Toll Free in USA 800-356-9526 · Phone 608-274-4330 · Fax 608-277-2516 · www.promega.com Printed in USA. Part# TM311... -
Page 33: D. Starting A Run
Figure 28. Defining a Luminescent Plate read. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA Toll Free in USA 800-356-9526 · Phone 608-274-4330 · Fax 608-277-2516 · www.promega.com Part# TM311 Printed in USA. -
Page 34: E. Kinetic Mode
Note: Kinetic Mode is not compatible with the 384-well plate format or Well Scan Mode for 6- to 48-well plate formats. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA Toll Free in USA 800-356-9526 · Phone 608-274-4330 · Fax 608-277-2516 · www.promega.com Printed in USA. Part# TM311... -
Page 35: F. Kinetic Parameters
Well: Shows the time taken to read each well using the defined parameters. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA Toll Free in USA 800-356-9526 · Phone 608-274-4330 · Fax 608-277-2516 · www.promega.com Part# TM311 Printed in USA. -
Page 36: G. Running The Standard Light Plate Protocol
>0.98 (Figure 31). If the results are <0.98 contact Promega Technical Services for assistance. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA Toll Free in USA 800-356-9526 · Phone 608-274-4330 · Fax 608-277-2516 · www.promega.com Printed in USA. Part# TM311... - Page 37 1.0 × 10 Figure 31. Calculating R value. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA Toll Free in USA 800-356-9526 · Phone 608-274-4330 · Fax 608-277-2516 · www.promega.com Part# TM311 Printed in USA. Page 36...
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Page 38: A. General Information
Figure 32. Optical Filters Wheel. Diagram shows the locations of standard and customizable filter paddles. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA Toll Free in USA 800-356-9526 · Phone 608-274-4330 · Fax 608-277-2516 · www.promega.com Printed in USA. Part# TM311... - Page 39 Avoid touching the filter itself. Figure 33. Absorbance filter paddle. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA Toll Free in USA 800-356-9526 · Phone 608-274-4330 · Fax 608-277-2516 · www.promega.com Part# TM311 Printed in USA.
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Page 40: C. Starting A Run
8. Place the sample plate on the Microplate Sample Tray. Well A1 must be at the top right corner. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA Toll Free in USA 800-356-9526 · Phone 608-274-4330 · Fax 608-277-2516 · www.promega.com Printed in USA. Part# TM311... - Page 41 Note: To prevent evaporation and spills, remove the sample plate from the instrument when the reading is complete. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA Toll Free in USA 800-356-9526 · Phone 608-274-4330 · Fax 608-277-2516 · www.promega.com Part# TM311 Printed in USA.
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Page 42: D. Ratiometric Assay
A manual change of the filter is not required. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA Toll Free in USA 800-356-9526 · Phone 608-274-4330 · Fax 608-277-2516 · www.promega.com Printed in USA. Part# TM311... -
Page 43: C. Customizable Absorbance Paddles With 260Nm And 280Nm Filters
Avoid touching the filter itself. Figure 36. Absorbance filter paddle. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA Toll Free in USA 800-356-9526 · Phone 608-274-4330 · Fax 608-277-2516 · www.promega.com Part# TM311 Printed in USA. -
Page 44: Fluorometer Operation
*Optical kits are named based on the color of the excitation wavelength. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA Toll Free in USA 800-356-9526 · Phone 608-274-4330 · Fax 608-277-2516 · www.promega.com Printed in USA. -
Page 45: C. Starting A Run
9. Touch the “Start” button. Figure 37. Defining a fluorescence plate read. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA Toll Free in USA 800-356-9526 · Phone 608-274-4330 · Fax 608-277-2516 · www.promega.com Part# TM311 Printed in USA. -
Page 46: D. Switching Fluorescence Optical Kits
The label should be facing up and outward. Figure 38. Installing a Fluorescence Optical Kit. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA Toll Free in USA 800-356-9526 · Phone 608-274-4330 · Fax 608-277-2516 · www.promega.com Printed in USA. Part# TM311... -
Page 47: Repeat Runs
“USB” button to transfer all files to a USB flash drive and view them on a computer. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA Toll Free in USA 800-356-9526 · Phone 608-274-4330 · Fax 608-277-2516 · www.promega.com Part# TM311 Printed in USA. -
Page 48: Data Management And Transfer
(upper right) to see a list of results files. The files will be sorted alphabetically and you can select a file to view. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA Toll Free in USA 800-356-9526 · Phone 608-274-4330 · Fax 608-277-2516 · www.promega.com Printed in USA. Part# TM311... - Page 49 Figure 42. Deleting data from the Results screen. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA Toll Free in USA 800-356-9526 · Phone 608-274-4330 · Fax 608-277-2516 · www.promega.com Part# TM311 Printed in USA.
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Page 50: B. Luminescence Data
Note: The maximum OD value of 5.0 means there was zero transmittance. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA Toll Free in USA 800-356-9526 · Phone 608-274-4330 · Fax 608-277-2516 · www.promega.com Printed in USA. -
Page 51: D. Fluorescence Data
Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA Toll Free in USA 800-356-9526 · Phone 608-274-4330 · Fax 608-277-2516 · www.promega.com Part# TM311 Printed in USA. -
Page 52: F. Transferring Data
5. Touch the “OK” button to export only the selected file(s). Touch the “Copy All Files” button to export all of the results files. The .csv formatted file(s) will be saved onto the USB flash drive in the folder named: Promega\Data 6. Move the USB flash drive to your local computer. -
Page 53: Connecting To A Pc
Documents\Promega\GloMax-Multi Plus\Protocols Event log = C:\Program Files\Promega\GloMax-Multi Plus\EventLog.txt Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA Toll Free in USA 800-356-9526 · Phone 608-274-4330 · Fax 608-277-2516 · www.promega.com Part# TM311 Printed in USA. Page 52... -
Page 54: D. Installing Microsoft .Net Framework Version 2.0 (X86)
Welcome screen, then select the “Next” button to continue. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA Toll Free in USA 800-356-9526 · Phone 608-274-4330 · Fax 608-277-2516 · www.promega.com Printed in USA. Part# TM311... - Page 55 10. A message reading “Your new hardware is installed and ready to use” will appear on the Taskbar to confirm successful installation. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA Toll Free in USA 800-356-9526 · Phone 608-274-4330 · Fax 608-277-2516 · www.promega.com Part# TM311 Printed in USA.
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Page 56: F. Connecting To A Pc Using A Usb Cable
-Multi+ Detection System, follow the instructions in Section 16.H. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA Toll Free in USA 800-356-9526 · Phone 608-274-4330 · Fax 608-277-2516 · www.promega.com Printed in USA. Part# TM311 Revised 5/10... -
Page 57: H. Disconnecting From A Pc
Please contact Promega Technical Services for details. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA Toll Free in USA 800-356-9526 · Phone 608-274-4330 · Fax 608-277-2516 · www.promega.com Part# TM311 Printed in USA. -
Page 58: B. Fluorescence Module
Optical Kit in the Fluorescence Module and keep the instrument door closed. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA Toll Free in USA 800-356-9526 · Phone 608-274-4330 · Fax 608-277-2516 · www.promega.com Printed in USA. Part# TM311... -
Page 59: C. Absorbance Module
(Figure 46). Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA Toll Free in USA 800-356-9526 · Phone 608-274-4330 · Fax 608-277-2516 · www.promega.com Part# TM311 Printed in USA. - Page 60 Absorbance Module into the threaded stainless steel post located on the left side of the instrument interior. See Figure 48. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA Toll Free in USA 800-356-9526 · Phone 608-274-4330 · Fax 608-277-2516 · www.promega.com Printed in USA. Part# TM311...
- Page 61 Figure 50. Detection Mode icon on the Instrument Status bar indicating the Absorbance Module is detected. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA Toll Free in USA 800-356-9526 · Phone 608-274-4330 · Fax 608-277-2516 · www.promega.com Part# TM311 Printed in USA.
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Page 62: D. Uv-Vis Absorbance Module
-Multi+ Detection System. Figure 52. Attaching the UV-Vis housing unit. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA Toll Free in USA 800-356-9526 · Phone 608-274-4330 · Fax 608-277-2516 · www.promega.com Printed in USA. Part# TM311... - Page 63 Instrument status bar of the Instrument Control screen (Figure 50). Figure 54. Spacer and 1.5 inch Allen head screw. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA Toll Free in USA 800-356-9526 · Phone 608-274-4330 · Fax 608-277-2516 · www.promega.com Part# TM311 Printed in USA.
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Page 64: Injector System Installation And Operation
Figure 56. GloMax -Multi+ Detection Injector System and accessories. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA Toll Free in USA 800-356-9526 · Phone 608-274-4330 · Fax 608-277-2516 · www.promega.com Printed in USA. Part# TM311 Revised 5/10... -
Page 65: B. Installation Procedure
Allen wrench. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA Toll Free in USA 800-356-9526 · Phone 608-274-4330 · Fax 608-277-2516 · www.promega.com Part# TM311 Printed in USA. Page 64... - Page 66 Press down firmly until the rod clicks in place. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA Toll Free in USA 800-356-9526 · Phone 608-274-4330 · Fax 608-277-2516 · www.promega.com Printed in USA. Part# TM311 Revised 5/10...
- Page 67 Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA Toll Free in USA 800-356-9526 · Phone 608-274-4330 · Fax 608-277-2516 · www.promega.com Part# TM311 Printed in USA.
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Page 68: C. Injector System Operation
A round flat-bottom bottle, a 50ml conical tube, a 15ml conical tube and a 15ml test tube. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA Toll Free in USA 800-356-9526 · Phone 608-274-4330 · Fax 608-277-2516 · www.promega.com Printed in USA. Part# TM311... - Page 69 2. From the Instrument Control screen, touch the “Read” button, and then touch the “Injector Setup” button. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA Toll Free in USA 800-356-9526 · Phone 608-274-4330 · Fax 608-277-2516 · www.promega.com Part# TM311 Printed in USA.
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Page 70: D. Using The Dispense Option
6. If a plate read is to follow Incubation step, select and define parameters. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA Toll Free in USA 800-356-9526 · Phone 608-274-4330 · Fax 608-277-2516 · www.promega.com Printed in USA. -
Page 71: Tools
From the Instrument Control screen, touch the “Tools”button to manage various aspects of the instrument (Figure 62). Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA Toll Free in USA 800-356-9526 · Phone 608-274-4330 · Fax 608-277-2516 · www.promega.com Part# TM311 Printed in USA. -
Page 72: A. System Information
Figure 62. The Tools Screen. Note the “Information” button, upper left. Figure 63. System Information. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA Toll Free in USA 800-356-9526 · Phone 608-274-4330 · Fax 608-277-2516 · www.promega.com Printed in USA. Part# TM311... -
Page 73: B. Event Log
-Multi+ Detection System Graphical User Interface software. The most current software versions can be obtained from Promega on a CD or via download. Please contact Promega Technical Services for more information. 1. Load the software onto a USB flash drive. -
Page 74: F. Updating Firmware
-Multi+ Detection System from an external PC. For setup instructions, see Section 16. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA Toll Free in USA 800-356-9526 · Phone 608-274-4330 · Fax 608-277-2516 · www.promega.com Printed in USA. Part# TM311... -
Page 75: Maintenance
Warning: If a fluid spill on the detector is suspected, immediately contact Promega Technical Services for cleaning instructions. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA Toll Free in USA 800-356-9526 · Phone 608-274-4330 · Fax 608-277-2516 · www.promega.com Part# TM311 Printed in USA. -
Page 76: C. Touch Screen Care
3. With the Microplate Sample Tray Cover disengaged, gently pull up to release it from the Tray assembly (Figure 65). Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA Toll Free in USA 800-356-9526 · Phone 608-274-4330 · Fax 608-277-2516 · www.promega.com Printed in USA. Part# TM311... - Page 77 Tray assembly. Figure 66. Aligning the Microplate Sample Tray Cover. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA Toll Free in USA 800-356-9526 · Phone 608-274-4330 · Fax 608-277-2516 · www.promega.com Part# TM311 Printed in USA.
- Page 78 Microplate Sample Plate Cover or force it to close. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA Toll Free in USA 800-356-9526 · Phone 608-274-4330 · Fax 608-277-2516 · www.promega.com Printed in USA.
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Page 79: E. Cleaning The Injectors
After each prime and flush sequence, discard any fluid from the Waste Collection Tray, and rinse it with deionized water. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA Toll Free in USA 800-356-9526 · Phone 608-274-4330 · Fax 608-277-2516 · www.promega.com Part# TM311 Printed in USA. -
Page 80: G. Replacing Injector Tips
10. Insert the tip into the injector tip holder on the Luminescence Module. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA Toll Free in USA 800-356-9526 · Phone 608-274-4330 · Fax 608-277-2516 · www.promega.com Printed in USA. -
Page 81: H. Inserting Injector Tip Assembly
7. Attach the adapter to the gray fitting by turning it clockwise and tighten by hand. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA Toll Free in USA 800-356-9526 · Phone 608-274-4330 · Fax 608-277-2516 · www.promega.com Part# TM311 Printed in USA. -
Page 82: K. Changing The Standard Light Plate Battery
(green light should appear). Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA Toll Free in USA 800-356-9526 · Phone 608-274-4330 · Fax 608-277-2516 · www.promega.com Printed in USA. Part# TM311... -
Page 83: Troubleshooting
Microplate Sample Tray was retracting Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA Toll Free in USA 800-356-9526 · Phone 608-274-4330 · Fax 608-277-2516 · www.promega.com Part# TM311 Printed in USA. Page 82... - Page 84 Promega Technical Services. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA Toll Free in USA 800-356-9526 · Phone 608-274-4330 · Fax 608-277-2516 · www.promega.com Printed in USA. Part# TM311 Revised 5/10...
- Page 85 Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA Toll Free in USA 800-356-9526 · Phone 608-274-4330 · Fax 608-277-2516 · www.promega.com Part# TM311 Printed in USA. Page 84...
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Page 86: B. Table Of Common Problems
70% ethanol and a damp cloth. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA Toll Free in USA 800-356-9526 · Phone 608-274-4330 · Fax 608-277-2516 · www.promega.com Printed in USA. Part# TM311 Revised 5/10... - Page 87 The battery is dead. See Section 20.K for information about changing the battery. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA Toll Free in USA 800-356-9526 · Phone 608-274-4330 · Fax 608-277-2516 · www.promega.com Part# TM311 Printed in USA.
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Page 88: Appendix
Stray Light: 0.002% at 560nm in clear bottom black-wall plates Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA Toll Free in USA 800-356-9526 · Phone 608-274-4330 · Fax 608-277-2516 · www.promega.com Printed in USA. Part# TM311... - Page 89 Ambient 20°–45°C ± 0.75°C • Heating Interval: 0.1–120 minutes Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA Toll Free in USA 800-356-9526 · Phone 608-274-4330 · Fax 608-277-2516 · www.promega.com Part# TM311 Printed in USA. Page 88...
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Page 90: B. Warranty And Service
1. Contact Promega Technical Services. 2. Carry out minor adjustments or tests as suggested by your Technical Services contact. 3. If it is determined that the instrument should be returned for repair, Promega Technical Services will arrange for service by an authorized GloMax ®... -
Page 91: C. Certificate Of Decontamination
Place: Signature: Name (block capital letters): Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA Toll Free in USA 800-356-9526 · Phone 608-274-4330 · Fax 608-277-2516 · www.promega.com Part# TM311 Printed in USA. Page 90 Revised 5/10... -
Page 92: D. Related Products
E2510 Luminescence Module *For Laboratory Use. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA Toll Free in USA 800-356-9526 · Phone 608-274-4330 · Fax 608-277-2516 · www.promega.com Printed in USA. Part# TM311 Revised 5/10 Page 91... - Page 93 G8211 Luminescence Module *For Laboratory Use. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA Toll Free in USA 800-356-9526 · Phone 608-274-4330 · Fax 608-277-2516 · www.promega.com Part# TM311 Printed in USA. Page 92 Revised 5/10...
- Page 94 G8531 Luminescence Module *For Laboratory Use. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA Toll Free in USA 800-356-9526 · Phone 608-274-4330 · Fax 608-277-2516 · www.promega.com Printed in USA. Part# TM311 Revised 5/10 Page 93...
- Page 95 All prices and specifications are subject to change without notice. Product claims are subject to change. Please contact Promega Technical Services or access the Promega online catalog for the most up-to-date information on Promega products.en are trademarks of Promega Corporation.
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