Operating The Injector System - Promega GloMax Operating Manual

4.B. Operating the Injector System
The injector system will inject reagent into all plate formats except 384-well plates.
Injectors can be used with all detection modes. They can also be used with all Kinetic, Loop and Shaking
protocols.
Warning: Make sure sufficient volume is available in the well to accept the injected volume. This is especially
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important for Kinetic and Loop protocols where multiple injection steps may occur and for protocols involving
shaking where liquid may spill out of the well if shaken too rapidly. Failure to do so may cause an overflow of
reagent and/or sample into adjacent wells or into the interior of the instrument.
You can define the injection volume and speed, delay time and integration time for each of the two injectors using
the Protocol builder from the 'Home' screen. This information is saved with each protocol.
The viscosity of solutions/reagents can affect the performance of the injectors.
If a protocol requires injectors, the injectors must first be primed with at least 500μl of the reagent to be injected.
Priming wets the fluid path and removes air bubbles to ensure delivery of an accurate injection volume for the
assay.
Priming is also required if an injection protocol has been interrupted for any reason (e.g., power failure).
You may need to optimize injection speed, depending on the solution/reagent you are working with. We
recommend using the default settings of 200µl per second as a starting point. From the Tools icon in the
menu bar, or accessed from the Tools button on the 'Settings' screen (Figure 62, Panel B), follow the guided
instructions to Prime, Reverse Purge and Flush the injectors.
Figure 94. Injector functions (prime, reverse purge and flush).
After finishing an assay, unused reagent in the fluid path can be retrieved. Select the option to Reverse Purge by
tapping the Start... button on the 'Injectors' screen. To prevent clogging of the fluid lines, flush after each use.
Note: The reagent container holder can accommodate four sizes of containers: a round flat-bottom bottle, a 50ml
conical tube, a 15ml conical tube and a 15ml test tube.
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TM397 · Revised 8/18