Promega GloMax Operating Manual page 60

Depending on the type of curve fit applied to the data, the following information will be created as a
result of pressing the Run button. The analysis formula is shown on the graph. Summarized statistical
information for each curve is included in the graph legend. Additional statistical information is included in
the data report.
Table 4. Summary of Statistical Information that is Generated from Performing an Analysis.
Analysis Curve
Fit Type
Linear Standard Curve
2nd Polynomial
3rd Polynomial
Power Regression
Four-Parameter Curve
Five-Parameter Curve
Dose Response EC /IC
50
Linear Response
Point to Point
Cubic Spline
Z´-Factor
Data from the plate read will be populated in the center of the wells on the plate map. To view results of a
single-read analysis, toggle the data displayed in the wells of the plate using the Data On/Off button at the
top of the screen (Figure 57).
To view results from a multi-read analysis, touch the associated data tab for each read located at the top of
the screen.
To view raw or analyzed data from dual-read result files, toggle the data view by pressing the Ratio On/Off,
Data 1 On/Off and Data 2 On/Off buttons at the top of the screen. To view raw or analyzed BRET data
from ratiometric NanoBRET™ result files, toggle the data view by pressing the Ratio On/Off,
Donor On/Off and Acceptor On/Off buttons at the top of the screen.
3. After the analysis has been run, touch the Graphing Tab on the right side of the screen to view the graph of
the results.
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Analysis Formula
Shown on Graph
y = f(x) = mx + b
f(x) = a + bx + cx
f(x) = a + bx + cx
f(x) = ax
b
(
T – B
f(x) = B +
1 + 10
(
T – B
f(x) = B +
(1 + 10
(logIC50–logx)S
(
T – B
f(x) = B +
50
1 + 10
y = f(x) = mx + b
No formula used
No formula used
(
Z´-Factor = 1 –
Data Shown
in Graph Legend
R
2
R
2 2
+ dx R
2 3 2
R
2
)
R , IC
2
(logIC50–logx)S
)
R , IC
2
)
S
)
R , IC
2
(logIC50–logx)S
R
2
No additional
information
included
No additional
information
included
)
+ s
3(s )
1 2
Z´-Factor Value
|µ – µ |
1 2
Additional Information
Included in Data Report
Slope, Intercept
A, B, C
A, B, C, D
A, B
Slope, Bottom, Top
50
Slope, Bottom, Top,
50 Asymmetry Factor (S)
Slope, Bottom, Top
50
Slope, Intercept
No additional information
included
No additional information
included
No additional information
included
TM397 · Revised 8/18
59