Promega GloMax Operating Manual page 45

3.F. Analysis of Results (continued)
Applying a Pre-Existing Analysis Template (continued)
3. When opening a result file containing two reads, the software will prompt the user to either take a ratio of
those two reads or proceed with analysis without taking a ratio. If prompted, select the formula mode before
proceeding to application of an analysis template:
Div (r1/r2) and Div (r2/r1): The user dictates which read is in the numerator and which read is in the
denominator. The ratio options are only available for read files containing two plate reads. After computing
ratio, select Continue to proceed with analysis of the ratiometric data. These options might be chosen if a
ratio of two absorbance measurements is desired or if one wishes to compute a ratio of an experimental and
control reporter prior to analysis.
Export Ratio: If no further analysis of the ratio is desired, selecting Export Ratio will export the ratio
directly to Excel
with no further analysis performed.
Multiple Reads: Choose this option if the multiple reads shall be analyzed separately without taking a
ratio. This option might be chosen if a multiplexed assay was performed consisting of two different plate
reads, with each to be analyzed separately.
Figure 45. Compute a ratio of a two-read result file, or select Multiple reads to analyze each read
separately.
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TM397 · Revised 8/18
. This option might be chosen if a ratio of two absorbance measurements is desired and
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