Promega GloMax Operating Manual page 57

3.F. Analysis of Results (continued)
Creating a New Analysis Template (continued)
12. Data normalization allows results to be expressed as either % Control or Fold-Change if desired.
Normalization can be performed either before or after an analysis has been completed. Normalization
requires the inclusion of a Control well type on the analysis template.
Note: If Control wells are to be included on the graph then they must first be assigned as a Known well type
within each experimental data set.
a. Highlight the wells to assign as a control. From the Well Type drop-down menu, assign those wells as
either Control 1 or Control 2 (Figure 55). Note: Data can be normalized to only one control at a time.
The Normalize button becomes active once a control has been assigned to the plate.
Figure 55. Control well assignment. A Control well type must be assigned in order to perform data
normalization to % Control or Fold Change. The Normalize button becomes active once a Control has been
assigned to the plate.
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TM397 · Revised 8/18 www.promega.com