Promega GloMax Operating Manual page 59

3.F. Analysis of Results (continued)
Performing an Analysis (continued)
Selecting Run will initiate the analysis. The average value of the blanks will be subtracted from the Known
and Unknown wells. Averages and Standard Deviations (if there are greater than three replicates of each
concentration) will be calculated for replicates and will be indicated in the data report. The analysis curve fit
will be applied and a graph will be generated of the analyzed data.
Figure 57. Once a template has been applied to the data, touch the Run button to analyze the results.
Note: Any changes made to the template (including the addition of Controls and performing data
normalization) will require re-running the analysis in order to view the changes on the graph.
2. Performing the analysis will generate a best-fit curve of the data shown on the graphing tab of the 'Analysis'
screen. The graphing tab will remain inactive until the Run button has been pressed.
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TM397 · Revised 8/18 www.promega.com