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Maxwell
®
Instrument Technical Manual
16 Forensic
Instructions for Use
of Product
AS3060
Printed in the USA
Revised 4/15
Part# TM321

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Table of Contents
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Summary of Contents for Promega Maxwell 16 Forensic

  • Page 1 Maxwell 16 Forensic ® Instrument Technical Manual Instructions for Use Printed in the USA of Product Revised 4/15 AS3060 Part# TM321...
  • Page 2: Table Of Contents

    7.A. Changing the Hardware ......................33 7.B. Changing the Instrument Firmware Configuration ..............37 Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 www.promega.com TM321 · Revised 4/15...
  • Page 3 12. Certificate of Decontamination ......................52 13. Summary of Changes ......................... 52 Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TM321 · Revised 4/15 www.promega.com...
  • Page 4: Introduction

    Sample Track is a communications program that allows downloading and printing of run data ® (see Technical Manual #TM314). Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 www.promega.com TM321 · Revised 4/15...
  • Page 5: Maxwell ® 16 Forensic Instrument Specifications

    16 Forensic Instrument is supplied preconfigured in the Forensic mode and with the LEV hardware. ® Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TM321 · Revised 4/15...
  • Page 6: Inspection

    If any item is damaged contact Promega Technical Services immediately. Standard accessories are shown in Figure 1. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 www.promega.com...
  • Page 7 Sample Track software. The Bar Code Reader and Bar Code Reader Cable are ® provided in a separate box. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TM321 · Revised 4/15 www.promega.com...
  • Page 8: Precautions

    • Equipment can be hazardous due to the use of chemical and biohazardous substances. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 www.promega.com TM321 · Revised 4/15...
  • Page 9 Please follow your institutional requirements for disposal destruction des accessoires of the accessories. 6687TA.eps Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TM321 · Revised 4/15 www.promega.com...
  • Page 10: Environmental Requirements (Operating, Shipping And Storage Conditions)

    16 Forensic Instrument. Figure 2. Front of Maxwell 16 Forensic Instrument. ® Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 www.promega.com TM321 · Revised 4/15...
  • Page 11 This picture shows the back of the instrument with the On/Off switch and the power cable connection. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TM321 · Revised 4/15...
  • Page 12 11.A). We recommend that you keep the SD card in the instrument to avoid misplacing it. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 www.promega.com...
  • Page 13 LEV cartridges is placed on the platform (rack not shown). Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TM321 · Revised 4/15...
  • Page 14: Unpacking And Setting Up The Maxwell ® 16 Forensic Instrument

    Important. Save the packaging material in case the instrument needs to be returned for service or repair later. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 www.promega.com...
  • Page 15: Removing The Magnetic Rod Assembly/Plunger Bar Platform Shipping Anchors

    Remove the UV Bulb from the box and wrapping. The bulb may rattle if Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TM321 ·...
  • Page 16: Maxwell ® Sample Track

    ® Connect the Bar Code Reader to the USB port on the side of the instrument. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 www.promega.com...
  • Page 17: Operational Setup

    Settings can be changed later if required (see Section 7.B). Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TM321 · Revised 4/15...
  • Page 18 Figure 12. Calibrating the Touch screen. To calibrate the screen touch the “+” symbols in the order they are presented. A confirmation screen will open. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 www.promega.com...
  • Page 19 Figure 14. Date/Time Setup screen. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TM321 · Revised 4/15...
  • Page 20 Bar code Input screen during run setup (Section 5), but you will not be able to scan bar code data for these options. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 www.promega.com...
  • Page 21 “Yes”, the Select User screen opens. The screen is initially populated with numbers. Select a number, then press the Run/Stop button. Figure 18. User Settings screen. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TM321 · Revised 4/15 www.promega.com...
  • Page 22 The Home screen contains the menu options that allow access to all functions available for the instrument and displays the hardware mode (SEV or LEV). Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 www.promega.com...
  • Page 23 (UV on; Section 8). This screen provides access to the service functions (Service mode; Section 11.B). Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TM321 · Revised 4/15...
  • Page 24: Hardware Mode

    A Verification screen indicates which protocol was chosen. Figure 21. Verification screen. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 www.promega.com TM321 · Revised 4/15...
  • Page 25: Setting Up Samples And Collecting Input Data

    Bulletin for detailed instructions regarding hardware mode, sample preparation, cartridge setup and purification method. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TM321 · Revised 4/15...
  • Page 26 Important. If you configured the instrument to collect run information, you must input the information to the Bar code Input screen chosen to perform the run. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 www.promega.com...
  • Page 27 Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TM321 · Revised 4/15...
  • Page 28 Select the Cart. ID option and the cartridge to which the sample will be added; select Manual. Use the keyboard to type in the data required. The data will appear next to Cart. ID. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 www.promega.com...
  • Page 29 For LEV runs, once all data have been verified, place the LEV Cartridge Rack into the instrument, and press the Run/Stop button to continue. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TM321 · Revised 4/15...
  • Page 30: Performing A Sample Purification Run

    If the instrument detects that any of these parts are not within calibration during a run, the method will pause and display Calibration Error. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 www.promega.com...
  • Page 31 (see Section 6). Figure 28. End screen. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TM321 · Revised 4/15...
  • Page 32: Post-Run Uv Light Treatment

    If Yes is selected, the File Output screen opens. Figure 29. File Output screen. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 www.promega.com...
  • Page 33: Data File Transfer At A Later Time

    16 Configuration screen. Here you can choose the parameter that you wish to change. ® Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TM321 · Revised 4/15...
  • Page 34: Changing The Hardware

    Unscrew the three thumbscrews (boxes in Figure 32) on top of the Magnetic Rod Assembly. Figure 32. SEV Magnetic Rod Assembly and thumbscrews. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 www.promega.com...
  • Page 35 Press firmly to place the LEV Magnetic Rod Assembly in position. It will fit tightly in place. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TM321 ·...
  • Page 36 Unscrew the three thumbscrews (boxes shown in Figure 36) on top of the Magnetic Rod Assembly. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 www.promega.com...
  • Page 37 Magnetic Rod Assembly in position. It will fit tightly into place. Figure 38. Installing the SEV Magnetic Rod Assembly. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TM321 · Revised 4/15...
  • Page 38: Changing The Instrument Firmware Configuration

    Important. You may need to scroll down the screen to see all options. Figure 39. Configuration screen. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 www.promega.com...
  • Page 39: Cleaning And Maintenance

    Important. Wear gloves or other protective means. If the instrument is used with biohazardous materials, dispose of any cleaning materials used in accordance with your institutional guidelines. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TM321 · Revised 4/15...
  • Page 40: Cleaning The Hardware

    UV treatment is not a substitute for cleaning. Using the UV lamp treatment alone may not provide sufficient decontamination. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 www.promega.com...
  • Page 41: Replacing The Uv Bulb

    (for example, Plungers in the tracks of the deck). Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TM321 · Revised 4/15...
  • Page 42 This means that there may be slight variations in run time. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 www.promega.com...
  • Page 43 It should be replaced. See Section 8.E. Figure 41. UV lamp error. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TM321 · Revised 4/15...
  • Page 44 (SEV or LEV). Refer to Section 7 for information on setting the instrument hardware mode. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 www.promega.com TM321 · Revised 4/15...
  • Page 45 If the calibration error persists, contact your local Promega representative for service. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TM321 · Revised 4/15...
  • Page 46 UV lamp comes on as part of the startup diagnostic tests. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 www.promega.com...
  • Page 47: Warranties, Service Agreements And Related Products

    Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TM321 · Revised 4/15...
  • Page 48: Warranty And Service Agreement Options

    Additionally the instrument is aligned and performance is verified. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 www.promega.com...
  • Page 49: Related Products And Instrument Accessories

    Home screen will open. If the firmware was loaded successfully, the main.bin file will be deleted from the SD card. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TM321 ·...
  • Page 50: Service

    Reset Name allows you to rename the instrument. Use the Run/Stop button to add the new name. Reset Wizard opens the Reset Wizard screen. • Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 www.promega.com TM321 · Revised 4/15...
  • Page 51: Instrument Return

    (see last page of this manual). Failure to complete and attach the Certificate of Decontamination will result in a decontamination charge. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TM321 · Revised 4/15...
  • Page 52: Instrument Disposal

    Contact your local Promega Representative for disposal of the instrument. Please follow your institutional requirements to handle the disposal of accessories. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 www.promega.com...
  • Page 53: Certificate Of Decontamination

    Promega online catalog for the most up-to-date information on Promega products. © 2014, 2015 Promega Corporation. All Rights Reserved. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TM321 · Revised 4/15...
  • Page 54 This instrument is certified by the undersigned to be free of radioactive contamination. Date: Place: Signature: Name (block capital letters): Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 www.promega.com TM321 · Revised 4/15...

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