Promega GloMax Operating Manual page 48

5. Touch the drop-down menu at the top left side of the screen to select the Curve Fit type for this template.
There are 11 analysis curve fit types to choose from. The tables below highlight each analysis curve fit type,
the function of each analysis and the formula used.
Table 2. Analysis Curve Fit Types That Allow Assignment of Known, Unknown, Blank, Control 1 and
Control 2 Well Types.
Analysis Curve Fit Type
Linear Standard Curve
2nd Polynomial
3rd Polynomial
Power Regression
Four-Parameter Curve
Five-Parameter Curve
Table 3. Analysis Curve Fit Types That Allow Assignment of Known, Blank, Control 1 and Control 2
Well Types.
Analysis Curve Fit Type
Dose Response EC /IC
50
Linear Response
Point to Point
Cubic Spline
Z´-Factor
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Function of Analysis Type
Linear regression; interpolate Unknown
concentration from curve
Nonlinear regression, second order; interpolate
Unknown concentration from curve
Nonlinear regression, third order; interpolate
Unknown concentration from curve
Nonlinear regression; interpolate Unknown
concentration from curve
Nonlinear regression; interpolate Unknown con-
centration from curve; EC
Nonlinear regression; interpolate Unknown con-
centration from curve; EC
Function of Analysis Type
Nonlinear regression; four-parameter curve fit;
EC /IC determination
50
50 50
Linear regression
Connects points together with a straight line
Connects points together with a smooth line
Calculates Z´-Factor
/IC determination
50 50
/IC determination
50 50
Analysis Formula
y = f(x) = mx + b
f(x) = a + bx + cx
2
f(x) = a + bx + cx + dx
2
f(x) = ax
b
(
T – B
f(x) = B +
1 + 10
(logIC50–logx)S
(
T – B
f(x) = B +
(1 + 10
(logIC50–logx)S
Analysis Formula
(
T – B
f(x) = B +
1 + 10
(logIC50–logx)S
y = f(x) = mx + b
No formula assigned
No formula assigned
(
3(s
Z´-Factor = 1 –
|µ
1
TM397 · Revised 8/18
3
)
)
)
S
)
)
+ s
)
1 2
– µ |
2
47