Promega GloMax Operating Manual page 31

3.C. Protocol Screen (continued)
Loop
Loops are used to perform a set of steps on a per-plate basis over a fixed time interval, then repeating the set of
steps a desired number of times.
Note: The interval (min) field defines the minimal interval of time for one loop iteration. If all of the operations
within the loop are completed before the specified interval time has passed, the software will wait for the specified
interval. If all of the operations take longer than the specified interval time, the software will continue to the next
iteration without waiting.
Note: Loops can be used with all detection modes, protocol functions and plate types. A maximum of 100 loops
can be performed within a single loop step.
Add the Loop step to the protocol by dragging and dropping the Loop icon into the protocol.
1.
Figure 31. Loop function.
2. Select the desired number of iterations.
3. If the same plate map will be used for each step of the protocol, lock the plate map in the upper right corner
to select the wells.
If a different plate map is to be used for various steps, unlock the plate map in the upper right corner, then
select the plate map icon within each step of the loop to select the desired wells.
4. Begin building the desired workflow by dragging and dropping the desired steps inside the Loop step. They
should be contained in the Loop step.
Note: If adding an injector step inside a loop function, ensure that there is sufficient space in the well to
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accommodate multiple additions of reagent.
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