Promega GloMax Operating Manual page 58

b. Touch the Normalize button to access three options for normalization (Figure 56). If only one
control has been assigned to the plate, that control is automatically chosen for normalization. If
more than one control is assigned to the plate, the user must specify which control to choose for
normalization (Figure 56).
i. Data: Undo any normalization performed on the plate.
% Control: Experimental wells will be expressed as a % response relative to the control wells.
ii.
Fold Change: Experimental wells will be expressed as fold response relative to the control
iii.
wells.
A.
Figure 56. Data normalization. Panel A. There are two options for data normalization, % Control and
Fold Change, and an option to undo normalization, Data. After selecting the normalization mode, normalization
will automatically assign the control that is present on the plate. Panel B. If there are two controls on the plate,
the user must specify which control to use for normalization.
c. Select OK to automatically normalize or undo normalization accordingly. If there are raw data present
in the wells, normalization of the results occurs once OK is pressed.
Once the user has defined the analysis template, touch the Save Template button. When prompted, enter
13.
a name for the template, and then select OK. The name of the analysis template will now appear in the title
bar and in the list of available analysis templates on the 'Templates' screen.
Performing an Analysis
Once a template has been assigned to the result file, touch the green Run button near the bottom of the
1.
left side of the screen to perform the analysis of samples (Figure 57). When the analysis is completed, an
"Analysis has successfully completed" message will appear on the screen. Select OK to view the analyzed
results.
Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516
www.promega.com
B.
57
TM397 · Revised 8/18