Optimize G Force And Flow Rate; Cell Size (D) - Thermo Scientific gibco CTS Rotea User Manual

Chapter 4 Protocol Building Blocks
4
Establish a fluidized bed

Optimize G force and flow rate

The two parameters most easily controlled during process development are the G force and the flow
rate. Entering the cell type and media used into the Rotea
predict the settings for both parameters.
Further optimisation of the settings can be performed based on observation of the fluidized bed using
the integrated camera and in-process sampling as adjustments are made to G force and flow rate.

Cell size (d)

• Small cells: Small cells take longer to settle with less opportunities for inter-cellular collisions
within the fixed CFC Chamber volume. Higher G forces, lower flow rates and a longer time are
usually required to establish a bed.
• Large cells: Large cells settle faster and the probability of inter-cellular collisions is higher.
Establishing a bed with large cells can occur quite quickly and less cells are required.
• Mixed cells: A mixed cell population comprising both small and large cells will tend to form
quickly, with the fluidized bed of large cells creating a stable environment for the small cells. The
challenge with mixed populations is finding settings that retain small cells without pelletizing the
large cells.
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Process Model application enables you to
CTS ™ Rotea ™ Counterflow Centrifugation System Process Design User Guide