Connect To Bioreactors - Thermo Scientific gibco CTS Rotea User Manual

Chapter 4 Protocol Building Blocks
4
Cell loading

Connect to bioreactors

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The Rotea instrument can be directly connected to many bioreactors provided there is a suitable fluid
connection and that priming is adequate (see "Prime remote sources"
If the bioreactor is a rigid vessel, it must be vented.
CAUTION! The user is responsible for ensuring that the risk of excessive pressure within the
total system is assessed and appropriate steps taken to prevent over-pressure conditions from
eventuating.
The tubing connecting the bioreactor chamber to Rotea
1/8" ID, (3.2 mm). If the tubing is >1/8" then bubbles can be difficult to drive out of the tubing. If
bubbles/air remain in the tubing, air will be drawn into the Rotea
process.
If the input line Bubble Sensor triggers during loading from a bioreactor, the entire input line may be
empty. This can represent a significant volume that requires re-filling before loading from the input line
can continue. To address this, a separate step to re-prime the input line can be added, either as a
standard step within the protocol or as a pre-prepared recovery step. One strategy is to connect line
G as close as possible to the input line. This enables wash buffer to be pumped through the CFC
Chamber and out through G to the bioreactor without disrupting the fluidized bed.
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instrument should ideally not be more than
CTS ™ Rotea ™ Counterflow Centrifugation System Process Design User Guide
on page 52).
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instrument when it starts the loading