Check Manual Clamps; Verify Reagent Connected And Not Clamped; Check For Closed Clamps - Thermo Scientific gibco CTS Rotea User Manual

Kit definition
The Single-Use Kit label includes the Valve ID for lines A to G (Note: Line H is not labelled). It is
recommended that reagent packs and other materials that will be attached to a Single-Use Kit, include
matching identification labels. Adding Bag volumes and descriptions when defining the Single-Use Kit
can also aid in kit preparation.
2D Barcode
The 2D Barcode defines the Single-Use Kit type. The 2D Barcode will be automatically checked upon
loading of the Single-Use Kit to the instrument to confirm that it matches the kit defined in the protocol.

Check manual clamps

A Pause step with the Skip button selected as the only Triggercan be added at the beginning of every
protocol to remind the operator to confirm that the manual clamps are open.

Verify reagent connected and not clamped

Create a pre-priming step with a Bubble Sensor Trigger set Dry to Wet and Skip button not selected.
Set centrifuge = 0 speed and pump at 50 mL/minute. The system will draw down on the reagent bag
until the Bubble Sensor indicates fluid is available to proceed.

Check for closed clamps

The Pressure Trigger provides a means to check each line before the protocol commits to processing
any product.
Add a Pressure Trigger - Pressure Rising to all priming steps as follows:
• Pressure 2 when priming lines B,C,D, E,F or H from bag A or G
• Pressure 1 when priming lines A or G from lines B, C, D, E, F or H
• Set the pressure value at 40 kPa.
Include a Pause on Trigger for the step with Skip button not selected. If the Pressure Trigger goes off
before the volume is delivered, the instrument will go into pause and cannot proceed until the clamp is
released and the target volume has been delivered.
CTS ™ Rotea ™ Counterflow Centrifugation System Process Design User Guide
Chapter 4 Protocol Building Blocks
Process integrity
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