The position of the normal distribution curves shows the population of each cell type relative to the zero
line.
In the above example, the monocytes will be retained in the CFC Chamber and most of the
lymphocytes will be elutriated along with the RBC's.
The % at each end of the normal distribution curves indicates the predicted percentage of cells that will
be retained (e.g. 10%) and elutriated (e.g. 90%) at the current settings.
The figures at the bottom of each graph reading from left to right are:
• maximum cell diameter (e.g. 9.0 micron)
• theoretical cell diameter at the zero line (e.g. 8.4 micron)
• minimum cell diameter (e.g. 5.0 micron)
CTS
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Rotea
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Counterflow Centrifugation System Process Design User Guide
Chapter 5 Rotea
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Process Model
Predict process settings
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