When used in conjunction with Thermo Scientific™ Pierce™ 1-Step Transfer Buffer, the Pierce G2 Fast Blotter is designed to provide transfer efficiency in 5-10 minutes that is equivalent to, or better than, traditional blotting techniques without the need for gel pre- equilibration.
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Technical Support email for U.S. and international customers outside Europe: pierce.ts@thermofisher.com Technical Support email for European customers: perbio.eurotech@thermofisher.com For more information about the Thermo Scientific Protein Biology product line, please visit thermoscientific.com/pierce / Thermo Scientific Pierce G2 Fast Blotter Thermo Scientific...
Instrument Overview Unpacking and Setup Instructions Unpack your new Pierce G2 Fast Blotter Control Unit and/or Cassette. Verify that all of the accessories listed below are included with your blotter package. Please note that the control unit and the cassette can be purchased separately or combined.
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3. Custom Methods Create, run and save a custom transfer method. 4. Setup Control audio settings, view software and hardware versions, and install software updates to the user interface. 5. Shutdown Safely shuts down the device. / Thermo Scientific Pierce G2 Fast Blotter Thermo Scientific...
China India South Korea Environmental Requirements For optimal performance, maintain a constant laboratory temperature range of 15-25°C (59- 77°F) with relative humidity between 20-70%. Do not place blotter in direct sunlight. Thermo Scientific / Thermo Scientific Pierce G2 Fast Blotter...
Figure 2.1. Blotter Control Unit front and side views detailing main components of control unit. Cathode Anode Cassette Contact Points Figure 2.2. Cassette view detailing the upper cathode, the lower anode and the electrical contacts that insert into the control unit contacts. / Thermo Scientific Pierce G2 Fast Blotter Thermo Scientific...
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Instrument Section 2 Cassette Control Unit Figure 2.3. Contol unit with cassette inserted. Power Switch Power Cord Connector Cooling Fan Figure 2.4. Rear view of control unit with cassette inserted. Thermo Scientific / Thermo Scientific Pierce G2 Fast Blotter...
The on-board computer boots up, the touchscreen displays the Thermo Scientific logo followed by “Welcome to the Pierce G2 Fast Blotter,” and then automatically moves to the Main Menu. The blotter is now ready for operation.
S E C T I O N 3 Fast Blotting: Constant Amps/Surface Area Fast blotting methods, such as the Pierce G2 Fast Blotter, require a high-current power supply and an optimized high ionic strength transfer buffer, such as Pierce 1-Step Transfer Buffer. By increasing the current, excellent transfer efficiency can be achieved in a much shorter time.
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(30-60 minutes). Traditional Semi-Dry: 1 mini gel, 25V constant, 1.0A limit 1.20 1.00 Volts 0.80 Amps 0.60 0.40 0.20 0.00 Time (minutes) Figure 3.2. Traditional semi-dry blotting protocol (volts vs amps). / Thermo Scientific Pierce G2 Fast Blotter Thermo Scientific...
If 0.83mm thick filter paper is not available, do not exceed 1.8mm total filter paper thickness for the bottom of the stack or 1.8mm total filter paper thickness for the top of the stack. Use the appropriate Thermo Scientific filter papers: Product No. 84873, 0.83mm thick, 7.0cm × 8.4cm (mini-size) Product No.
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5. Slide cassette into the control unit. 6. Select Pre-Programmed Methods in the Main Menu. 7. Using the touchpad, select the number of gels and gel size (mini or midi) you wish to transfer: / Thermo Scientific Pierce G2 Fast Blotter Thermo Scientific...
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When continuously processing multiple samples at ~5A for a maximum of 2 hours, allow the cassette to cool for 30 minutes or use multiple cassettes to avoid excessive cassette heating. Thermo Scientific / Thermo Scientific Pierce G2 Fast Blotter...
Ensure there is a 1cm space around all stack edges to allow any gases to escape during transfer. / Thermo Scientific Pierce G2 Fast Blotter Thermo Scientific...
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7. Select the number of gels and gel size (mini or midi) you wish to transfer: a. 1 mini-sized gel b. 2 mini-sized gels or 1 midi-sized gel c. 3 mini-sized gels d. 4 mini-sized gels or 2 midi-sized gels Thermo Scientific / Thermo Scientific Pierce G2 Fast Blotter...
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9. Select the Start button to begin transfer. After running at high current, the anode and cathode plates can become hot. Use caution when separating the gels and stacks from the plates. / Thermo Scientific Pierce G2 Fast Blotter Thermo Scientific...
1. Pressing the Select Constant (V or A) button will toggle the constant variable parameter from amps to volts or volts to amps. 2. Highlight the variable for change and press the Up or Down arrow to raise or lower the selected variable’s value. Thermo Scientific / Thermo Scientific Pierce G2 Fast Blotter...
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15 characters to identify the new custom method. Press Done and then Start to begin the method. The custom method will be saved in Custom Methods in the Main Menu. / Thermo Scientific Pierce G2 Fast Blotter Thermo Scientific...
3. Highlight the variable for change and press the Up or Down arrow to raise or lower the selected variable’s value. Thermo Scientific / Thermo Scientific Pierce G2 Fast Blotter...
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15 characters to identify the new custom method. Press Done and then Start to begin the method. The custom method will be saved in Custom Methods in the Main Menu. / Thermo Scientific Pierce G2 Fast Blotter Thermo Scientific...
45-60 minutes. Current normally spikes and then quickly drops to a very low level, while voltage reaches its maximum (25 volts). Thermo Scientific / Thermo Scientific Pierce G2 Fast Blotter...
2. Wipe the exterior surfaces of the control unit and cassette. Use caution to avoid touching the LCD touchscreen with anything other than a screen-cleaning wipe. 3. Ensure that the cooling fan vent is free of dust and debris. / Thermo Scientific Pierce G2 Fast Blotter Thermo Scientific...
If the cathode has excessive salt build-up, use a mild acetic acid solution (< 5%) to remove the residue. 2. Rinse with deionized water and stand parts in a rack to dry. Thermo Scientific / Thermo Scientific Pierce G2 Fast Blotter...
15:85 ratio before equilibrating filter paper and proteins to PVDF proteins (< 25kDa) to PVDF membrane (e.g., 15mL of ethanol with 85mL of Pierce membrane 1-Step Transfer Buffer) / Thermo Scientific Pierce G2 Fast Blotter Thermo Scientific...
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