Table of Contents
Advertisement
Quick Links
Advertisement
Table of Contents
Related Manuals for Thermo Scientific Pierce G2
Summary of Contents for Thermo Scientific Pierce G2
- Page 1 Thermo Scientific Pierce G2 Fast Blotter Version 1 May 2013...
- Page 2 © 2013 Thermo Fisher Scientific Inc. All rights reserved. All trademarks are the property of Thermo Fisher Scientific Inc. and its subsidiaries. Specifications, terms and pricing are subject to change. Not all products are available in all countries. Please consult your local sales representative for details. Thermo Scientific...
-
Page 3: Table Of Contents
Section 5 ........................28 Maintenance ........................ 28 Cleaning the Blotter LCD Touchscreen ..................28 Cleaning the Blotter Housing and the Cassette ................. 28 Cleaning the Anode and Cathode Plates ................... 29 Troubleshooting ......................... 30 Thermo Scientific / Thermo Scientific Pierce G2 Fast Blotter... -
Page 5: Section 1
When used in conjunction with Thermo Scientific™ Pierce™ 1-Step Transfer Buffer, the Pierce G2 Fast Blotter is designed to provide transfer efficiency in 5-10 minutes that is equivalent to, or better than, traditional blotting techniques without the need for gel pre- equilibration. - Page 6 Technical Support email for U.S. and international customers outside Europe: pierce.ts@thermofisher.com Technical Support email for European customers: perbio.eurotech@thermofisher.com For more information about the Thermo Scientific Protein Biology product line, please visit thermoscientific.com/pierce / Thermo Scientific Pierce G2 Fast Blotter Thermo Scientific...
-
Page 7: Instrument Overview
Instrument Overview Unpacking and Setup Instructions Unpack your new Pierce G2 Fast Blotter Control Unit and/or Cassette. Verify that all of the accessories listed below are included with your blotter package. Please note that the control unit and the cassette can be purchased separately or combined. - Page 8 3. Custom Methods Create, run and save a custom transfer method. 4. Setup Control audio settings, view software and hardware versions, and install software updates to the user interface. 5. Shutdown Safely shuts down the device. / Thermo Scientific Pierce G2 Fast Blotter Thermo Scientific...
-
Page 9: Section 2
Table 2.1. Control unit dimensions. Dimensions Inches Centimeters Width 6.44 16.4 Depth 10.88 27.6 Height 6.06 15.40 Table 2.2. Cassette dimensions. Dimensions Inches Centimeters Width 10.13 25.7 Depth 10.94 27.8 Height 1.38 Thermo Scientific / Thermo Scientific Pierce G2 Fast Blotter... -
Page 10: Electrical Requirements
Table 2.3. Blotter electrical ratings. Electrical Parameter Rating Supply Voltage (VAC) 120-240 Frequency (Hz) 50-60 Power Rating (W) Fuse (power center) T4AL,250V, 4A / Thermo Scientific Pierce G2 Fast Blotter Thermo Scientific... -
Page 11: Environmental Requirements
China India South Korea Environmental Requirements For optimal performance, maintain a constant laboratory temperature range of 15-25°C (59- 77°F) with relative humidity between 20-70%. Do not place blotter in direct sunlight. Thermo Scientific / Thermo Scientific Pierce G2 Fast Blotter... -
Page 12: Blotter Images With Feature Locations
Figure 2.1. Blotter Control Unit front and side views detailing main components of control unit. Cathode Anode Cassette Contact Points Figure 2.2. Cassette view detailing the upper cathode, the lower anode and the electrical contacts that insert into the control unit contacts. / Thermo Scientific Pierce G2 Fast Blotter Thermo Scientific... - Page 13 Instrument Section 2 Cassette Control Unit Figure 2.3. Contol unit with cassette inserted. Power Switch Power Cord Connector Cooling Fan Figure 2.4. Rear view of control unit with cassette inserted. Thermo Scientific / Thermo Scientific Pierce G2 Fast Blotter...
-
Page 14: Start Up
The on-board computer boots up, the touchscreen displays the Thermo Scientific logo followed by “Welcome to the Pierce G2 Fast Blotter,” and then automatically moves to the Main Menu. The blotter is now ready for operation. -
Page 15: Section 3
S E C T I O N 3 Fast Blotting: Constant Amps/Surface Area Fast blotting methods, such as the Pierce G2 Fast Blotter, require a high-current power supply and an optimized high ionic strength transfer buffer, such as Pierce 1-Step Transfer Buffer. By increasing the current, excellent transfer efficiency can be achieved in a much shorter time. - Page 16 (30-60 minutes). Traditional Semi-Dry: 1 mini gel, 25V constant, 1.0A limit 1.20 1.00 Volts 0.80 Amps 0.60 0.40 0.20 0.00 Time (minutes) Figure 3.2. Traditional semi-dry blotting protocol (volts vs amps). / Thermo Scientific Pierce G2 Fast Blotter Thermo Scientific...
-
Page 17: Pierce 1-Step Transfer Buffer
If 0.83mm thick filter paper is not available, do not exceed 1.8mm total filter paper thickness for the bottom of the stack or 1.8mm total filter paper thickness for the top of the stack. Use the appropriate Thermo Scientific filter papers: Product No. 84873, 0.83mm thick, 7.0cm × 8.4cm (mini-size) Product No. - Page 18 5. Slide cassette into the control unit. 6. Select Pre-Programmed Methods in the Main Menu. 7. Using the touchpad, select the number of gels and gel size (mini or midi) you wish to transfer: / Thermo Scientific Pierce G2 Fast Blotter Thermo Scientific...
- Page 19 When continuously processing multiple samples at ~5A for a maximum of 2 hours, allow the cassette to cool for 30 minutes or use multiple cassettes to avoid excessive cassette heating. Thermo Scientific / Thermo Scientific Pierce G2 Fast Blotter...
-
Page 20: Traditional Semi-Dry Transfer Method
Ensure there is a 1cm space around all stack edges to allow any gases to escape during transfer. / Thermo Scientific Pierce G2 Fast Blotter Thermo Scientific... - Page 21 7. Select the number of gels and gel size (mini or midi) you wish to transfer: a. 1 mini-sized gel b. 2 mini-sized gels or 1 midi-sized gel c. 3 mini-sized gels d. 4 mini-sized gels or 2 midi-sized gels Thermo Scientific / Thermo Scientific Pierce G2 Fast Blotter...
- Page 22 9. Select the Start button to begin transfer. After running at high current, the anode and cathode plates can become hot. Use caution when separating the gels and stacks from the plates. / Thermo Scientific Pierce G2 Fast Blotter Thermo Scientific...
-
Page 23: Section 4
1. Pressing the Select Constant (V or A) button will toggle the constant variable parameter from amps to volts or volts to amps. 2. Highlight the variable for change and press the Up or Down arrow to raise or lower the selected variable’s value. Thermo Scientific / Thermo Scientific Pierce G2 Fast Blotter... - Page 24 15 characters to identify the new custom method. Press Done and then Start to begin the method. The custom method will be saved in Custom Methods in the Main Menu. / Thermo Scientific Pierce G2 Fast Blotter Thermo Scientific...
-
Page 25: Creating A New Custom Method
3. Highlight the variable for change and press the Up or Down arrow to raise or lower the selected variable’s value. Thermo Scientific / Thermo Scientific Pierce G2 Fast Blotter... - Page 26 15 characters to identify the new custom method. Press Done and then Start to begin the method. The custom method will be saved in Custom Methods in the Main Menu. / Thermo Scientific Pierce G2 Fast Blotter Thermo Scientific...
-
Page 27: Recommended Maximum Watts/Hour Limit
45-60 minutes. Current normally spikes and then quickly drops to a very low level, while voltage reaches its maximum (25 volts). Thermo Scientific / Thermo Scientific Pierce G2 Fast Blotter... -
Page 28: Section 5
2. Wipe the exterior surfaces of the control unit and cassette. Use caution to avoid touching the LCD touchscreen with anything other than a screen-cleaning wipe. 3. Ensure that the cooling fan vent is free of dust and debris. / Thermo Scientific Pierce G2 Fast Blotter Thermo Scientific... -
Page 29: Cleaning The Anode And Cathode Plates
If the cathode has excessive salt build-up, use a mild acetic acid solution (< 5%) to remove the residue. 2. Rinse with deionized water and stand parts in a rack to dry. Thermo Scientific / Thermo Scientific Pierce G2 Fast Blotter... -
Page 30: Troubleshooting
15:85 ratio before equilibrating filter paper and proteins to PVDF proteins (< 25kDa) to PVDF membrane (e.g., 15mL of ethanol with 85mL of Pierce membrane 1-Step Transfer Buffer) / Thermo Scientific Pierce G2 Fast Blotter Thermo Scientific... - Page 31 thermoscientific.com/pierce © 2013 Thermo Fisher Scientific Inc. All rights reserved. Facebook is a registered trademark o f Facebook, Inc. All other trademarks are the property of Thermo Fisher Scientific Inc. and its subsidiaries. Life Science Research Africa/Belgium/Europe/Middle East USA +815-968-0747 or +800-874-3723 Email: perbio.euromarketing@thermofisher.com thermoscientific.com/pierce +32 53 85 71 84...
Need help?
Do you have a question about the Pierce G2 and is the answer not in the manual?
Questions and answers