Thermo Scientific gibco CTS Rotea User Manual page 31

Chapter 3 Rotea Protocol Builder Application
3
Step view
5. Harvest– is used to deliver a high concentration of cells to valves E, F or H with less carryover of
the buffer. During harvest the valve for the destination bag is kept closed until the volume entered
in Cone to Valve Volume has been diverted past the valve. The valve for the destination bag is
then opened, dispensing the concentrate. The default Cone to Valve Volume is set at 0.5 mL and
can be optimized to suit the different valves. During a Harvest step the OD Sensor will always
record the optical density and calculate the concentrate volume. This is stored as OD Sensor
Volume in Volume Register and can be used as a trigger.
31
CTS ™ Rotea ™ Counterflow Centrifugation System Process Design User Guide