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Chapter 4 Protocol Building Blocks
4
Cell loading
Re-use input bags
Rinsing and re-priming input bags can enable re-use of the bag for subsequent steps in a protocol.
Option 1: Two-port bag used for recirculating bed formation.
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With the cells loaded in the fluidized bed, wash
buffer is delivered through the bed and into the input
bag through line G. This fluid is then used to "rinse"
the input bag.
Note: This is also a good wash strategy if there is
potential for the wash step to disrupt the bed due to
differential media densities.
The loading conditions are re-established, and the
rinse fluid is drawn into the fluidized bed until the
bubble sensor triggers.
Note: It may be necessary to re-prime the input line
(D in this case) prior to loading the rinse fluid.
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Counterflow Centrifugation System Process Design User Guide
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