Wash Cells And Buffer Exchange; Overview - Thermo Scientific gibco CTS Rotea User Manual

Wash cells and buffer exchange

Overview

Counterflow centrifugation depends on the formation of a fluidized bed where cells are surrounded by,
and in intimate contact with, the media flowing through the bed. This means that cells in the fluidized
bed are rapidly exposed to new media resulting in very fast and efficient buffer exchange using a small
volume of wash media. The basic cell washing steps using counterflow centrifugation are as follows:
CTS ™ Rotea ™ Counterflow Centrifugation System Process Design User Guide
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Chapter 4 Protocol Building Blocks
Wash cells and buffer exchange
Details
Create a fluidized bed of cells in the original media.
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