Normal Step; Speed Settings - Thermo Scientific gibco CTS Rotea User Manual

Chapter 4 Protocol Building Blocks
4
Concentrate Recovery

Normal step

The simplest method for Concentrate Recovery is to program a Normal step where the pump is
reversed, and set to deliver a Target Volume to an output bag using Wash Buffer as the source fluid.

Speed settings

Different circumstances and cell types require process specific settings but a good starting point in
process development is to simply reverse the pump flow with the G force constant and the flow rate
equal to or slightly greater than in the prior step.
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CTS ™ Rotea ™ Counterflow Centrifugation System Process Design User Guide