Thermo Scientific A44769 System Manuals

Manuals and User Guides for Thermo Scientific A44769 System. We have 2 Thermo Scientific A44769 System manuals available for free PDF download: User Manual

Thermo Scientific A44769 User Manual (128 pages)

Counterflow Centrifugation System Process Design

Brand: Thermo Scientific | Category: Laboratory Equipment | Size: 9 MB

Table of Contents
3
CHAPTER 1 Product Information

7
CHAPTER 2 Software Installation

12
- Software Installation
  12
CHAPTER 3 Rotea Protocol Builder Application

17
- Create or Open a Protocol
  17
- Define Kit
  18
- CTS ™ Rotea ™ Single-Use Kit Diagram
  19
- Cell Type
  21
- Description
  21
- Kit Type
  22
- Long Description
  22
- Step View
  23
- Define Fluid Path and Direction of Flow
  24
- Step Details
  25
- Step Type
  27
- Trigger
  32
- Volume
  33
- Timer
  35
- Pressure 1 and Pressure 2
  36
- Bubble Sensor Input and Bubble Sensor Output
  37
- Data Entry
  38
- List View
  39
- Step Summary
  40
- Edit Using List View
  42
- Loops
  42
- Repeat Count
  43
- Bubble Sensor Not Triggered
  44
- Process Counter
  44
- Timer (Seconds) and Timer (Minutes)
  45
- Skip Button Triggered
  45
- Editing Loops
  46
CHAPTER 4 Protocol Building Blocks

47
- Priming
  47
- Overview
  47
- Bubbles
  47
- Priming Volumes
  48
- Basic Priming Steps
  49
- Prime Remote Sources
  52
- Manage Air in the System
  53
- Establish a Fluidized Bed
  53
- Overview
  53
- The Seven Parameters for Establishing a Fluidized Bed
  55
- Optimize G Force and Flow Rate
  56
- Cell Size (D)
  56
- Media Density (Ρ)
  57
- Cell Concentration
  57
- Establish a Bed in Recirculation
  57
- Establish a Bed Without Recirculation
  59
- What a Good Bed Looks Like
  60
- Establish a Bed: Troubleshooting
  62
- Cell Loading
  64
- Overview
  64
- Cells Loaded into the CFC Chamber
  64
- Bed Disruption During Loading
  65
- Cell Separation During Loading
  65
- Re-Use Input Bags
  66
- Refill the Bubble Trap
  69
- Connect to Bioreactors
  70
- Wash Cells and Buffer Exchange
  71
- Overview
  71
- Media Selection
  72
- Wash to Similar Density Media
  72
- Wash to more Dense Media
  72
- Wash to Lower Density Media
  73
- Defensive Washing Strategies
  73
- Elutriation
  77
- Overview
  77
- Improve Cell Viability
  79
- Leukopak Processing
  79
- Typical Leukaphoresis Composition
  80
- Lentivirus Clarification
  81
- Separate Cells from Microcarriers
  81
- Elutriation Techniques
  82
- Concentrate Recovery
  85
- Overview
  85
- Normal Step
  86
- Speed Settings
  86
- Pause Step
  87
- Harvest Step
  88
- Harvest Using OD Sensor
  90
- Multi-Bite Processing
  92
- Overview
  92
- Determine the Number of Cells Per Bite
  92
- Maximize Throughput
  93
- Process Integrity
  94
- Overview
  94
- CTS ™ Rotea ™ Single-Use Kit
  94
- Check Manual Clamps
  95
- Verify Reagent Connected and Not Clamped
  95
- Check for Closed Clamps
  95
CHAPTER 5 Rotea

96
- Process Model
  96
- Counter Flow Centrifugation Forces
  96
- Predict Process Settings
  98
- Select and Format Media
  100
- Create Custom Media
  102
- Model Particle Behavior
  103
- Change and Delete Particles from a Model
  106
- Create Custom Particles
  107
- Edit Custom Particles and Custom Media
  107
CHAPTER 6 Rotea

109
- Simulation Software
  109
- Rotea ™ Simulation Software
  109
CHAPTER 7 Design New Protocols

115
- Plan the Kit Configuration
  115
- Plan the Protocol
  115
- Prepare for the First Trial Run
  116
- Water Runs
  116
- First Run with Cells
  116
- Analyze the Outcome
  116
- Refine the Protocol to Achieve Target Cell Outcomes
  116
- Add Process Integrity Features
  117
APPENDIX A Safety

118
- Symbols on this Instrument
  118
- Standard Safety Symbols
  119
- Control and Connection Symbols
  119
- Conformity Symbols
  119
- Safety Information for Instruments Not Manufactured by Thermo Fisher Scientific
  121
- Instrument Safety
  121
- General
  121
- Physical Injury
  122
- Electrical Safety
  122
- Cleaning and Decontamination
  123
- Safety and Electromagnetic Compatibility (EMC) Standards
  123
- Safety Standards
  123
- EMC Standards
  124
- Environmental Design Standards
  124
- Chemical Safety
  125
- Biological Hazard Safety
  126
APPENDIX B Documentation and Support

127
- Customer and Technical Support
  127
- Limited Product Warranty
  127

Thermo Scientific A44769 User Manual (132 pages)

Counterflow Centrifugation System

Brand: Thermo Scientific | Category: Laboratory Equipment | Size: 15 MB

Table of Contents
3
CHAPTER 1 Product Information

7
- Product Description
  7
- Features
  7
- Contents
  7
- System Overview
  8
  - Instrument Description
    8
  - Instrument Dimensions
    10
  - Working Space
    10
  - Instrument Weight
    10
  - Instrument Properties
    11
  - Push Button Controls
    13
  - CTS ™ Rotea ™ Single-Use Kit Schematic
    14
  - Wetted Components
    15
  - Allowed Chemicals for Wetted Surfaces
    15
CHAPTER 2 Installation and Setup

16
- Packaging Contents
  16
- Instrument Installation
  16
- Power Instrument "On
  18
- Power Instrument "Off
  19
- Installation
  20
- First Time Setup
  24
- Download Protocols
  27
  - Create Protocols
    27
  - Load a Protocol
    27
  - Rotea ™ GUI Dashboard
    31
  - User Management
    32
- Uninstall
  37
- Logging
  38
CHAPTER 3 Basic Instrument Operation

41
- Open the Door
  41
- Kit Preparation, Loading and Removal
  43
  - Kit Configuration
    43
  - Pre-Assembly of Processing Kit
    45
  - Connecting Vessels, Bags and Reagents
    46
  - Bubble Trap
    47
  - Kit Installation
    48
  - Initiate a Protocol
    54
- Run a Protocol
  61
  - Rotea ™ GUI Navigation and Instrument Operation
    61
  - Error Messages
    67
- Kit Removal
  69
- Aseptic Disconnection of the Kit Components
  74
- System Settings
  75
  - Instrument Firmware
    75
  - Opc-Ua
    80
  - Settings
    81
  - Licenses
    82
- Calibration
  83
CHAPTER 4 OPC-UA Interface

87
- Introduction
  87
- Definitions
  87
- Scope of System
  88
  - Instrument Hardware Configuration
    88
  - Single-Use Kit
    88
  - Protocol File
    89
  - Protocol Metadata
    89
- MES Interface
  89
  - Background
    89
  - Electronic Communications
    90
  - Communications Protocol
    90
- MES - Commands and Reports Overview
  91
  - Enable OPC-UA
    92
  - Log in to Instrument
    92
  - Load Protocol
    92
  - Load Kit
    93
  - Transfer Metadata
    93
  - Initialize Protocol on to Instrument
    93
  - Start/Stop/Pause/Resume Protocol
    93
  - Instrument Run Status
    94
  - MES Batch History Data
    94
  - Alarm Recovery Tools
    95
- OPC-UA Interface
  96
  - Commands OPC-UA to Rotea ™ Application
    96
  - Reports Rotea ™ Application to OPC-UA
    97
- Use Case Sequence Diagrams
  104
CHAPTER 5 Maintenance

110
- User Maintenance Schedule
  110
- Instrument Cleaning
  110
- Inspect Casing
  110
- Open the Door Without Power
  111
- Power and Fuses
  112
  - Power Supply
    112
  - Fuses
    112
CHAPTER 6 Troubleshooting

113
CHAPTER 7 Compliance

115
CHAPTER 8 Safety Instructions

116
- Labels
  116
- Emergency Procedures
  116
  - Instrument Emergency Stop Events
    116
  - Emergency Shut down
    117
  - Restart after Shut down
    117
  - Protocol Recovery Process
    118
- Warnings and Precautions
  119
  - Warnings
    119
  - Cautions
    120
APPENDIX A Safety

121
- Symbols on this Instrument
  121
  - Standard Safety Symbols
    122
  - Control and Connection Symbols
    122
  - Conformity Symbols
    122
- Safety Information for Instruments Not Manufactured by Thermo Fisher Scientific
  124
- Instrument Safety
  124
  - General
    124
  - Physical Injury
    125
  - Electrical Safety
    125
  - Cleaning and Decontamination
    126
- Safety and Electromagnetic Compatibility (EMC) Standards
  126
  - Safety Standards
    126
  - EMC Standards
    127
  - Environmental Design Standards
    127
- Chemical Safety
  128
- Biological Hazard Safety
  129
APPENDIX B Documentation and Support

130
- Customer and Technical Support
  130
- Limited Product Warranty
  130

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Thermo Scientific A44769 System Manuals

Thermo Scientific A44769 User Manual (128 pages)

Table of Contents

CHAPTER 1 Product Information

CHAPTER 2 Software Installation

CHAPTER 3 Rotea Protocol Builder Application

CHAPTER 4 Protocol Building Blocks

CHAPTER 5 Rotea

CHAPTER 6 Rotea

CHAPTER 7 Design New Protocols

APPENDIX A Safety

APPENDIX B Documentation and Support

Thermo Scientific A44769 User Manual (132 pages)

Table of Contents

CHAPTER 1 Product Information

CHAPTER 2 Installation and Setup

CHAPTER 3 Basic Instrument Operation

CHAPTER 4 OPC-UA Interface

CHAPTER 5 Maintenance

CHAPTER 6 Troubleshooting

CHAPTER 7 Compliance

CHAPTER 8 Safety Instructions

APPENDIX A Safety

APPENDIX B Documentation and Support

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