Thermo Scientific gibco CTS Rotea User Manual page 104

Chapter 5 Rotea ™ Process Model
5
Predict process settings
™
The Rotea Process Model assumes that the cell diameter is a normal distribution between the
maximum and minimum cell diameter defined for each cell type.
Prior to inputting values for the centrifuge speed and flow rate, the normal distribution curve for the
particles will be shaded grey and positioned centrally about the zero line.
Adjust the centrifuge speed (G) and flow rate (mL/min) to model the behaviour of the cells within the
CFC Chamber under the different processing conditions until you achieve the desired outcome:
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CTS ™ Rotea ™ Counterflow Centrifugation System Process Design User Guide