Thermo Scientific gibco CTS Rotea User Manual page 76

Chapter 4 Protocol Building Blocks
4
Wash cells and buffer exchange
(continued)
76
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CTS ™ Rotea
Re-establish the fluidized bed using recirculation and
then load the cells, directing the output to the waste
bag.
Note: It may be necessary to re-prime the input line
prior to this step.
A final wash of the cells using the new media can
now be completed with low risk of cell loss.
™ Counterflow Centrifugation System Process Design User Guide
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