Chapter 4 Protocol Building Blocks
4
Priming
Priming volumes
When priming it is important that enough volume is pumped through the system to remove all air
from the CFC Chamber, Bubble Trap and fluid lines. The table shows the approximate volumes of the
Single-Use Kit components to assist with calculating the correct priming volumes.
Note: Always include additional volume in each priming step to ensure adequate priming.
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Counterflow Centrifugation System Process Design User Guide