Prime Remote Sources - Thermo Scientific gibco CTS Rotea User Manual

Chapter 4 Protocol Building Blocks
4
Priming

Prime remote sources

When drawing cell product from a bioreactor or large vessel the supply line can often contain too much
air volume for the bubble trap to accept before fluid arrives. A lot of bioreactors also use large bore
tubing (i.e. >1/8") that can allow fluid to flow past bubbles.
Priming bags that use large bore tubing requires a two-step priming process.
Pre-Prime Large Bore Line
The goal is to draw the air into the system and pull it into the Bubble Trap – or through it to another bag.
Set a fluid path from the input source using the large bore line to your waste bag (in this example D to
A). Set a Bubble Sensor (Dry to Wet) trigger, and check that Pause on Bubble is not selected. This is
to ensure control over the volume you pump in the next step. The flow rate does not affect this step, so
a faster flow rate e.g. 50–100 mL/min, can be used. The centrifuge should be set to a low G force, to
avoid issues if the bubbles travel through the centrifuge.
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CTS ™ Rotea ™ Counterflow Centrifugation System Process Design User Guide