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Concentrate Recovery
Overview
The primary objective of Concentrate Recovery is to recover all cells contained within the fluidized
bed. Additional objectives might include:
• Minimize carryover of wash buffer
• Maximize cell concentration
• Achieve a target cell concentration
• Achieve a target volume
• Dilute with media in preparation for the subsequent process
Concentrate recovery is achieved by reversing the pump with the CFC Chamber still spinning.
The fluidized bed emerges from the CFC Chamber as a concentrated "slug" of cells in the media used
for the prior step e.g. wash buffer, EP buffer or culture media.
Lines E, F and H are specifically concentrate output lines since cells can be delivered with minimum
dead volume or disruption of the concentrate.
Note: The Rotea
controlled volume.
The following sections describe the tools available for programming and optimizing the concentrate
recovery sequence.
CTS
™
Rotea
™
Counterflow Centrifugation System Process Design User Guide
™
system has been designed to optimize cell recovery with high cell density in a small
Chapter 4 Protocol Building Blocks
Concentrate Recovery
4
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